European Journal of Chemistry 2015, 6(2), 178-182 | doi: https://doi.org/10.5155/eurjchem.6.2.178-182.1234 | Get rights and content






  OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS

Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study


Samah Abd Elsabour Mohammed (1,*) , Mohammed El-Dardiri (2) , Mona Ahmed Elhabak (3) , Khaled Ibrahim Abu Zaid (4)

(1) National Organization of Drug Control and Research, Giza, 11843, Egypt
(2) Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
(3) Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
(4) Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
(*) Corresponding Author

Received: 24 Dec 2014 | Revised: 14 Feb 2015 | Accepted: 14 Feb 2015 | Published: 30 Jun 2015 | Issue Date: June 2015

Abstract


Metformin hydrochloride was quantified in human plasma by Ultra Performance Liquid Chromatography with Tandem Mass spectrometric determination (UPLC-MS/MS). The mobile phase used was (Water + 0.1% Formic acid) and (Acetonitrile + 0.1% Formic acid) in the ratio of 80:20 (v:v). An Acquity UPLC HSS T3 1.8 um 2.1×50 mm column was used. The detection was performed on a mass spectrometer (ESI+) using chlorpheniramine as an internal standard. The method was fully validated and it was applied to bioequivalence study of metformin hydrochloride in two brands of the drug with relative bioequivalence of 94.7%.


Keywords


Plasma; Validation; UPLC/MS/MS; Bioequivalence; Chlorpheneramine; Metformin hydrochloride

Full Text:

PDF /    /


DOI: 10.5155/eurjchem.6.2.178-182.1234

Links for Article


| | | | |

| | | | | | |

Related Articles




Article Metrics

This Abstract was viewed 539 times | PDF Article downloaded 296 times

Funding information


MEPACO Pharmaceutical Company (Arab Company for Pharmaceutical and Medicinal Plant), Elsharkya, Egypt

References

[1]. O'Neil, M. J.; Heckelman P. E.; Koch C. B.; Roman K. J. The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals, 4th Edition, Merck & Co., Inc., Whitehouse Station, NJ, 2012.

[2]. Goodarzi, M. O.; Bryer-Ash, M. Diabetes Obes. Metab. 2005, 7(6), 654-665.
http://dx.doi.org/10.1111/j.1463-1326.2004.00448.x

[3]. Holt, R. I. G.; Cockram, C.; Flyvbjerg, A.; Goldstein, B. J. Text book for diabetes, 4th edition, John Wiley & Sons, 2009.

[4]. Liu, A.; Coleman, S. P. J. Chromatogr. B 2009, 877(29), 3695-3700.
http://dx.doi.org/10.1016/j.jchromb.2009.09.020

[5]. Chen, L.; Zhou, Z.; Shen, M.; Ma, A. J. Chromatogr. Sci. 2011, 49, 94-100.
http://dx.doi.org/10.1093/chrsci/49.2.94

[6]. Chen, X.; Gu, Q.; Qiu, F.; Dafa, Z. D. J. Chromatogr. B 2004, 802(2), 377-381.
http://dx.doi.org/10.1016/j.jchromb.2003.12.017

[7]. Marlice, A.; Marques, S.; Soares, A.; Pinto, O. W.; Werneck, B. P. T. J. Chromatogr. B 2007, 852, 308-316.
http://dx.doi.org/10.1016/j.jchromb.2007.01.030

[8]. Ding, C. G.; Zhou, Z.; Ge, Q.; Zhi, X.; Ma, L. L. Biomed. Chromatogr. 2007, 21(2), 132-138.
http://dx.doi.org/10.1002/bmc.723

[9]. Ranetti, M. C.; Ionescu, M.; Hinescu, L.; Ionica, E.; Anuta, V.; Ranetti, A. E.; Stecoza, C. E.; Mircioiu, C. Farmacia 2009, 57(6), 728-735.

[10]. Bonde, S. L.; Bhadane, R. P.; Gaikwad, A.; Katale, D.; Gavali, S.; Narendiran, A. S. A. Int. J. Pharm. Sci. 2013, 3, 463-470.

[11]. Amini, H; Ahmadiani, A.; Gazerani, P. J. Chromatogr. B 2005, 824(1-2), 319-322.
http://dx.doi.org/10.1016/j.jchromb.2005.07.009

[12]. Jian, W.; Ying, W. G.; Jing-kai, G. U.; Yan, W. Chem. Res. Chinese. 2005, 21(2), 246-250.

[13]. Zhang, W.; Han, F.; Zhao, H.; Lin, Z. J.; Huang, Q. M.; Weng, N. Biomed. Chromatogr. 2011, 26(10), 1163-1169.
http://dx.doi.org/10.1002/bmc.2673

[14]. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Guidance for Industry-Bioanalytical Method validation, MD, 2001.

How to cite


Mohammed, S.; El-Dardiri, M.; Elhabak, M.; Abu Zaid, K. Eur. J. Chem. 2015, 6(2), 178-182. doi:10.5155/eurjchem.6.2.178-182.1234
Mohammed, S.; El-Dardiri, M.; Elhabak, M.; Abu Zaid, K. Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study. Eur. J. Chem. 2015, 6(2), 178-182. doi:10.5155/eurjchem.6.2.178-182.1234
Mohammed, S., El-Dardiri, M., Elhabak, M., & Abu Zaid, K. (2015). Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study. European Journal of Chemistry, 6(2), 178-182. doi:10.5155/eurjchem.6.2.178-182.1234
Mohammed, Samah, Mohammed El-Dardiri, Mona Ahmed Elhabak, & Khaled Ibrahim Abu Zaid. "Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study." European Journal of Chemistry [Online], 6.2 (2015): 178-182. Web. 16 Feb. 2020
Mohammed, Samah, El-Dardiri, Mohammed, Elhabak, Mona, AND Abu Zaid, Khaled. "Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study" European Journal of Chemistry [Online], Volume 6 Number 2 (30 June 2015)

DOI Link: https://doi.org/10.5155/eurjchem.6.2.178-182.1234

| | | | |

| | | | | |

Save to Zotero Save to Mendeley



European Journal of Chemistry 2015, 6(2), 178-182 | doi: https://doi.org/10.5155/eurjchem.6.2.178-182.1234 | Get rights and content

Refbacks

  • There are currently no refbacks.




Copyright (c)




© Copyright 2019  Atlanta Publishing House LLC All Right Reserved.

The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.

Copyright 2019 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 4614 Lavista road, Tucker, GA, 30084, USA. Registered in USA.

hatay escort hatay escort corlu escort corum escort burgaz escort giresun escort aydin escort ordu escort erzincan escort hatay escort sivas escort rize escort edirne escort aksaray escort kibris escort isparta escort erzurum escort tekirdag escort usak escort urfa escort kastamonu escort kibris escort manisa escort giresun escort urfa escort nevsehir escort sivas escort yalova escort ordu escort hatay escort yalova escort amasya escort kayseri escort ordu escort maras escort canakkale escort yalova escort balikesir escort manisa escort urfa escort mugla escort trabzon escort bolu escort corlu escort diyarbakir escort isparta escort kutahya escort elazig escort erzurum escort sakarya escort afyon escort kutahya escort konya escort agri escort cesme escort sinop escort sivas escort konya escort kibris escort adapazari escort luleburgaz escort adana escort kibris escort rize escort sakarya escort alanya escort isparta escort burdur escort konya escort bitlis escort canakkale escort sivas escort amasya escort mus escort aydin escort van escort yalova escort kastamonu escort mardin escort bolu escort afyon escort sakarya escort isparta escort tokat escort trakya escort bayburt escort urfa escort mardin escort