OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Ultra-performance liquid chromatography coupled to electrospray ionization-tandem mass spectrometric method for simultaneous determination of acemetacin in presence of its metabolite indomethacin and degradation products
Maha Mahmoud Abou-El Alamin (1,*)
(1) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(*) Corresponding Author
Received: 02 Apr 2016 | Revised: 23 Apr 2016 | Accepted: 29 Apr 2016 | Published: 30 Jun 2016 | Issue Date: June 2016
Selective and sensitive ultra-performance liquid chromatography-tandem mass spectrometric technique (UPLC-MS/MS) was investigated for simultaneous determination of acemetacin (ACM) in presence of its metabolite (indomethacin) and degradation products in 3 min run time. The column employed was a Hypersil gold 50 mm × 2.1 mm (1.9 μm) with an isocratic mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile (10:90, v:v) with flow rate 250 µL/min. Detection of the cited drug was carried out using multiple reaction monitoring (MRM) mode on a triple quadrupole mass spectrometer coupled with electrospray ionization (ESI) m/z 416.44 → 139.24 for ACM and m/z 256.31 → 167.00 for diphenhydramine internal standard. Various parameters were studied; mobile phase composition, flow rate, rate of fragmentation, rate of collision and mode of ionization. Good linear relationship was obtained in concentration range 8.0-500.0 ng/mL (r = 0.9994). The method was validated (linearity, range, precision, accuracy, limit of quantification and limit of detection) according to ICH guidelines and there is no significance difference between the proposed method and the reference HPLC method regarding accuracy and precision. The simplicity and sensitivity of this method allows its use as stability indicating method.
Links for Article
| | | | |
| | | | | | |
Article MetricsThis Abstract was viewed 557 times | PDF Article downloaded 216 times
Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
. Acemetacin, Wikipedia, the free encyclopaedia, https://en.wikipedia.org/wiki/Acemetacin
. Ozbakan, H.; Palabiyik, I. M.; Caglayan, M. G.; Onur, F. Turk. J. Pharm. Sci. 2009, 6, 163‐176.
. Naguib, I. A.; Abdel-Aleem, E. A.; Zaazaa, H.; Hussein, E. A. Eur. J. Chem. 2014, 5, 219‐226.
. Walash, M. I.; Belal, F.; Tolba, M. M.; Halawa, M. I. Sep. Sci. Tech. 2015, 50, 1403-1412.
. Kessiba, A. M.; Hegazy, M. A. M.; Abdelkawy, M. M.; El Gendy, A. E. Eur. J. Chem. 2015, 6(4), 422‐429.
. Ban, E.; Cho, J. H.; Jang, D. J.; Piao, X. L.; Kim, C. K. J. Liq. Chrom. Rel. Tech. 2005, 28, 1593‐1604.
. Reguera, C.; Arcos, M. J.; Ortiz, M. C. Talanta 1998, 46, 1493‐1505.
. Reguera, C.; Ortiz, M. C.; Arcos, M. J. Electroanal. 2002, 14, 1699‐1706.
. British Pharmacopoeia; British Pharmacopoeia Commission; The stationary Office, 2012.
. Mowaka, S.; Mohamed, D. RSC Adv. 2015, 5, 60467-60481.
. ICH, Harmonized Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology, Q2(R1), Parent Guidelines on Methodology, 2005.
How to cite
DOI Link: https://doi.org/10.5155/eurjchem.7.2.213-216.1434
| | | | |
| | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2016, 7(2), 213-216 | doi: https://doi.org/10.5155/eurjchem.7.2.213-216.1434 | Get rights and content
- There are currently no refbacks.
© Copyright 2010 - 2020 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2020 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.