European Journal of Chemistry 2011, 2(1), 109-112 | doi: https://doi.org/10.5155/eurjchem.2.1.109-112.147 | Get rights and content






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Voltammetric and viscometric studies of flavonoids interactions with DNA at physiological conditions


Syed Tajammul Hussain (1,*) , Asima Siddiqa (2)

(1) National Centre for Physics, Quaid-i-Azam University, Islamabad, 44000, Pakistan
(2) National Centre for Physics, Quaid-i-Azam University, Islamabad, 44000, Pakistan
(*) Corresponding Author

Received: 04 Jun 2010 | Accepted: 08 Dec 2010 | Published: 28 Mar 2011 | Issue Date: March 2011

Abstract


Cyclic voltammetric behaviour of three flavonoids: quercetin, morin and rutin was investigated for their interaction with DNA at pH = 4.7 and pH = 7.4 at body temperature (310 K), using glassy carbon electrode. The diffusion coefficients of the free and DNA bound forms of the flavonoids were evaluated using Randles–Sevcik equation. The binding parameters like binding constant, binding site size and binding free energy were also determined from voltammetric data. Moreover, the binding modes of flavonoids with DNA were evaluated from viscommetric analysis. A comparatively high value of binding constant, binding site size and diffusion coefficients for quercetin reveals its stronger binding with DNA under physiological conditions. The negative values of ∆G indicate the spontaneity of binding of flavonoids with DNA.

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Keywords


DNA; Flavonoids; Cyclic voltammetry; Binding constant; Gibbs free energy; Binding site size

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DOI: 10.5155/eurjchem.2.1.109-112.147

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Funding information


Quaid-i-Azam University

Citations

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[1]. Petar M. Mitrasinovic
Sequence-Dependent Binding of Flavonoids to Duplex DNA
Journal of Chemical Information and Modeling  55(2), 421, 2015
DOI: 10.1021/ci5006965
/


[2]. Anna Masek, Ewa Chrzescijanska, Marian Zaborski
Electrooxidation of morin hydrate at a Pt electrode studied by cyclic voltammetry
Food Chemistry  148, 18, 2014
DOI: 10.1016/j.foodchem.2013.10.003
/


[3]. D.A. Chernikov, T.A. Shishlyannikova, A.V. Kashevskii, B.N. Bazhenov, A.V. Kuzmin, A.G. Gorshkov, A.Y. Safronov
Some peculiarities of taxifolin electrooxidation in the aqueous media: The dimers formation as a key to the mechanism understanding
Electrochimica Acta  271, 560, 2018
DOI: 10.1016/j.electacta.2018.03.179
/


[4]. Stalin Selvaraj, Sridharan Krishnaswamy, Venkappayya Devashya, Swaminathan Sethuraman, Uma Maheswari Krishnan
Synthesis, characterization and DNA binding properties of rutin–iron complex
RSC Advances  2(7), 2797, 2012
DOI: 10.1039/c2ra01319c
/


[5]. Jing-Jing Zhou, Yu Mei, Zhiquan Pan, Hong Zhou
Structure, DNA binding and cleavage of a new Zn(II)Mn(II) macrocyclic complex
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy  99, 329, 2012
DOI: 10.1016/j.saa.2012.08.029
/


[6]. Esam Arafa Gomaa, Amr Negm, Mohamed Abdel Khalek Tahoon
Study of redox behavior of Cu(II) and interaction of Cu(II) with lysine in the aqueous medium using cyclic voltammetry
European Journal of Chemistry  7(3), 341, 2016
DOI: 10.5155/eurjchem.7.3.341-346.1471
/


References

[1]. Battin, E. E.; Perron, N. R.; Brumaghim, J. L. Inorg. Chem. 2006, 45, 499-501.
doi:10.1021/ic051594f
PMid:16411681

[2]. Ader, P.; Wessmann, A.; Wolffram, S. Free Rad. Biol. and Med. 2000, 28, 1056-1060.
doi:10.1016/S0891-5849(00)00195-7

[3]. Russo, A.; Acquaviva, R.; Campisi, A. Cell Biol. Toxicol. 2000, 16, 91-95.
doi:10.1023/A:1007685909018
PMid:10917564

[4]. Ren, W.; Qiao, Z.; Wang, H.; Zhu, L.; Zhang, L. Med. Res. Rev. 2003, 23, 519-523.
doi:10.1002/med.10033
PMid:12710022

[5]. Middleton, E.; Kandaswami, C.; Theoharides T. C. Pharmacol. Rev. 2000, 52, 673-680.
PMid:11121513

[6]. Pietta, P. G. J. Nat. Prod. 2000, 63, 1035-1039.
doi:10.1021/np9904509
PMid:10924197

[7]. Hollman, P. C.; Katan, M. B. Food Chem. Toxicol. 1999, 37, 937-941.
doi:10.1016/S0278-6915(99)00079-4

[8]. Vijayalakshmi, R.; Kantimathi, M.; Subramanian, V. Biochem. Biophys. Res. Commun. 2000, 271, 731-735.
doi:10.1006/bbrc.2000.2707
PMid:10814531

[9]. Shah, A.; Qureshi, R.; Kausar, N.; Haque, S. Anal. Sci. 2008, 24, 1437-1441.
doi:10.2116/analsci.24.1437
PMid:18997372

[10]. Zhu, Z.; Li, C.; Li, N. Q. Microchem. J. 2002, 71, 57-62.
doi:10.1016/S0026-265X(01)00118-7

[11]. Cao, Y.; He, X. Spectrochim. Acta A 1998, 54, 883-884.
doi:10.1016/S1386-1425(97)00277-1

[12]. Wang, F.; Xu, Y.; Zhao, J.; Shengshui, H. Bioelectrochem. 2007, 70, 356-361.
doi:10.1016/j.bioelechem.2006.05.003
PMid:16787762

[13]. Kanakis, C. D.; Tarantilis, P. A.; Polissou, M. S.; Diamantoglou, S. J. Biomol. Struc. Dyn. 2005, 22, 739-742.

[14]. Kang, J.; Li, Z.; Lu, X. J. Pharm. Biomed. Anal. 2006, 40, 1166-1170.
doi:10.1016/j.jpba.2005.08.030
PMid:16256288

[15]. Aslanoglu, M. Anal. Sci. 2006, 22, 439-445.
doi:10.2116/analsci.22.439
PMid:16733319

[16]. Janjua, N. K.; Siddiqa, A.; Yaqub, A.; Sabahat, S.; Qureshi, R.; Haque, S. Spectrochim. Acta. A 2009, 74, 1135-1140.
doi:10.1016/j.saa.2009.09.022
PMid:19836298

[17]. Aslanoglu, M. Turk. J. Chem. 2005, 29, 477-481.

[18]. Veal, J. M.; Rill, R. L. Biochem. 1991, 30, 1132-1136.
doi:10.1021/bi00218a035
PMid:1989681

How to cite


Hussain, S.; Siddiqa, A. Eur. J. Chem. 2011, 2(1), 109-112. doi:10.5155/eurjchem.2.1.109-112.147
Hussain, S.; Siddiqa, A. Voltammetric and viscometric studies of flavonoids interactions with DNA at physiological conditions. Eur. J. Chem. 2011, 2(1), 109-112. doi:10.5155/eurjchem.2.1.109-112.147
Hussain, S., & Siddiqa, A. (2011). Voltammetric and viscometric studies of flavonoids interactions with DNA at physiological conditions. European Journal of Chemistry, 2(1), 109-112. doi:10.5155/eurjchem.2.1.109-112.147
Hussain, Syed, & Asima Siddiqa. "Voltammetric and viscometric studies of flavonoids interactions with DNA at physiological conditions." European Journal of Chemistry [Online], 2.1 (2011): 109-112. Web. 24 Oct. 2020
Hussain, Syed, AND Siddiqa, Asima. "Voltammetric and viscometric studies of flavonoids interactions with DNA at physiological conditions" European Journal of Chemistry [Online], Volume 2 Number 1 (28 March 2011)

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DOI Link: https://doi.org/10.5155/eurjchem.2.1.109-112.147

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