European Journal of Chemistry

New HPLC method for determination of cystatin C biomarker in human blood

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Published: Dec 31, 2017
DOI: https://doi.org/10.5155/eurjchem.8.4.378-383.1658
Keywords:
Cystatin C, Biomarker, Kidney failure, Chromatography, Glomerular filtration rate, Determination of cystatin C
Section
Research Article
Issue
Vol. 8 No. 4 (2017): December 2017
Dates
Received 2017-10-04
Accepted 2017-10-22
Published 2017-12-31


Main Article Content

Othman Ibrahem AlMusaimi
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
Abd-Alhakeem Hasan Abu-Nawwas
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
Danah Mahdi AlShaer
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
Nabeel Yousef Khaleel
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia

Abstract

A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r2 = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively.


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AlMusaimi, O. I.; Abu-Nawwas, A.-A. H.; AlShaer, D. M.; Khaleel, N. Y. New HPLC Method for Determination of Cystatin C Biomarker in Human Blood. Eur. J. Chem. 2017, 8, 378-383.

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