European Journal of Chemistry 2020, 11(1), 80-83 | doi: | Get rights and content


Spectrum subtraction method for simultaneous determination of acetaminophen and caffeine in panadol extra dosage forms

Mahmoud Mohammed Sebaiy (1) orcid , Amr Abd El-Hakeem Mattar (2,*) orcid

(1) Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
(2) Pharmaceutical Medicinal Chemistry Department, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo 11829, Egypt
(*) Corresponding Author

Received: 30 Dec 2019 | Revised: 15 Feb 2020 | Accepted: 25 Feb 2020 | Published: 31 Mar 2020 | Issue Date: March 2020


A simple, specific, accurate and precise spectrophotometric method was established for simultaneous determination of acetaminophen and caffeine in pure form and in their pharmaceutical formulation commercially known as Panadol Extra®. Spectrum subtraction has been used in simultaneous determination of both drugs without prior separation. Spectrum subtraction method parameters were validated according to ICH guidelines in which accuracy, precision, repeatability and robustness were found in accepted limits (98-102%). The linearity range was 7.5-45 µg/mL for caffeine and 4-22 µg/mL for acetaminophen with correlation coefficients ≥0.9990 for both drugs. Advantages and disadvantages of spectrum subtraction were discussed and statistical comparison between the proposed method and the reference one was performed.


Caffeine; ICH guidelines; Acetaminophen; Spectrophotometric; Spectrum subtraction; Statistical comparison

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DOI: 10.5155/eurjchem.11.1.80-83.1956

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How to cite

Sebaiy, M.; Mattar, A. Eur. J. Chem. 2020, 11(1), 80-83. doi:10.5155/eurjchem.11.1.80-83.1956
Sebaiy, M.; Mattar, A. Spectrum subtraction method for simultaneous determination of acetaminophen and caffeine in panadol extra dosage forms. Eur. J. Chem. 2020, 11(1), 80-83. doi:10.5155/eurjchem.11.1.80-83.1956
Sebaiy, M., & Mattar, A. (2020). Spectrum subtraction method for simultaneous determination of acetaminophen and caffeine in panadol extra dosage forms. European Journal of Chemistry, 11(1), 80-83. doi:10.5155/eurjchem.11.1.80-83.1956
Sebaiy, Mahmoud, & Amr Abd El-Hakeem Mattar. "Spectrum subtraction method for simultaneous determination of acetaminophen and caffeine in panadol extra dosage forms." European Journal of Chemistry [Online], 11.1 (2020): 80-83. Web. 29 May. 2020
Sebaiy, Mahmoud, AND Mattar, Amr. "Spectrum subtraction method for simultaneous determination of acetaminophen and caffeine in panadol extra dosage forms" European Journal of Chemistry [Online], Volume 11 Number 1 (31 March 2020)

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