European Journal of Chemistry

Composition and content of total phenolics and flavonoids, and antioxidant activity of Trigonella isthmocarpa



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Serife Selma Uras Gungor
Sevda Guzel Ozay
Ahmet Ilcim
Gamze Kokdil

Abstract

The aqueous methanolic extracts obtained from aerial parts and seeds of T. isthmocarpa Boiss. & Bal. from Turkey were analyzed by reversed-phase HPLC for their phenolic acid and flavonoid composition. Total phenolic content was determined using the Folin-Ciocalteu assay, while total flavonoid content was measured spectrometrically with AlCl3 assay. Primary antioxidant activity in terms of free radical scavenging activities of both extracts was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest total flavonoid and total phenolic content was determined in the extract of aerial parts (830.8 mg/100 g and 877.9 mg gallic acid equivalent/100 g, respectively). The extracts of aerial parts and seeds had the high antioxidant activity (87.2 % and 78.2%, respectively). Gallic acid, caffeic acid, luteolin, apigenin and kaempferol were detected as phenolic compounds in both extracts by HPLC methods. In addition, an isoflavone, formononetin was determined in the extract from aerial parts of the plant. The present results suggest that T. isthmocarpa have shown potential a source of natural antioxidants.

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Gungor, S. S. U.; Ozay, S. G.; Ilcim, A.; Kokdil, G. Composition and Content of Total Phenolics and Flavonoids, and Antioxidant Activity of Trigonella Isthmocarpa. Eur. J. Chem. 2013, 4, 7-9.

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References

[1]. Bown, D. Encyclopedia of Herbs & Their Uses, 1st edition, Darling Kindersley Limited, 2002.

[2]. Evans, W. C. Trease and Evans Pharmacognosy, 15th edition, University of Nottingham, 2002.

[3]. Townsend, C. C. Leguminosae. In Flora of Iraq, The University Press, 1974.
PMCid:1355892

[4]. Basch, E.; Ulbricht, C.; Kuo, G.; Szapary, P.; Smith, M. Altern. Med. Rev. 2003, 8, 20-26.
PMid:12611558

[5]. Srinivasan, K. Food Rev. Int. 2006, 22, 203-224.
http://dx.doi.org/10.1080/87559120600586315

[6]. Pandian, R. S.; Anuradha, C. V.; Viswanathan, P. J. Ethnopharmacol. 2002, 81, 393¬-397.

[7]. Toppo, F. A.; Akhand, R.; Pathak A. K. Asian J. Pharm. Clin. Res. 2009, 2(4), 28-38.

[8]. Yaday, R.; Kaushik, R.; Gupta, D. Inter. J. Eng. Res. Appl. 2011, 1, 32-35.

[9]. Bown Yuldashev, M. P. Chem. Nat. Compd. 2002, 38, 291-292.
http://dx.doi.org/10.1023/A:1020448531522

[10]. Kawashyt, S. A.; Abdalla, M. F.; El Din, E. M. G.; Saleh, N. A. M. Biochem. Syst. Ecol. 1998, 26, 851-856.
http://dx.doi.org/10.1016/S0305-1978(96)00101-9

[11]. Huber-Morath, A. Trigonella L. in: Davis P. H. (ed.), Flora of Turkey and The East Aegean Island, Edinburgh University Press, 1969.

[12]. Davis, P. H.; Mill, R. R.; Tan, K. Flora of Turkey and The East Aegean Island, Edinburgh University Press, 1988.

[13]. Guner, A.; Ozhatay, N.; Ekim, T.; Baser, K. H. C. Leguminosae. Flora of Turkey and The East Aegean Islands (Supplement 2), Edinburgh University Press, 2000.

[14]. Gikas, E.; Bazoti, F. N.; Papadopoulos, N.; Alesta, A.; Economou, G.; Tsarbopoulos, A. Anal. Lett. 2011, 44, 1463-1472.
http://dx.doi.org/10.1080/00032719.2010.512683

[15]. Kim, D. O.; Jeong, S. W.; Lee, C. Y. Food Chem. 2003, 81, 321-326.
http://dx.doi.org/10.1016/S0308-8146(02)00423-5

[16]. Sunhasree, B.; Baskar, R.; Keerthana, R. L.; Susan, R. L.; Rajasekaran, P. Food Chem. 2009, 115, 1213-1220.
http://dx.doi.org/10.1016/j.foodchem.2009.01.029

[17]. Wang, S.; Di, D.; Liu, X.; Jiang, S. J. Liq. Chromatogr. Rel. Technol. 2007, 30, 1991-1999.
http://dx.doi.org/10.1080/10826070701386637

[18]. Khoschsorur, G. A.; Erwa, W. J. Chromatogr. B 2004, 799, 355-360.
http://dx.doi.org/10.1016/j.jchromb.2003.10.074

[19]. Yen, G. C.; Duht, P. D. J. Agr. Food Chem. 1994, 42, 629-632.
http://dx.doi.org/10.1021/jf00039a005

[20]. Mariod, A. A.; Matthaus, B.; Hussein I. H. Int. J. Food Sci. Tech. 2008, 43, 921-926.
http://dx.doi.org/10.1111/j.1365-2621.2007.01543.x

[21]. Zheng, C. D.; Li, G.; Li, H. Q.; Xu, X. J.; Gao, J. M.; Zhang, A. L. Nat. Prod. Commun. 2010, 5, 1759-1765.
PMid:21213975

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