European Journal of Chemistry 2014, 5(1), 81-84. doi:10.5155/eurjchem.5.1.81-84.881

A simple spectrophotometric method for determination of thiamine (vitamin B1) in pharmaceuticals


Khairia Mohammed Al-Ahmary (1,*)

(1) Chemistry Department, Sciences Faculty for Girls, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
(*) Corresponding Author

Received: 17 Jul 2013, Accepted: 18 Aug 2013, Published: 31 Mar 2014

Abstract


A simple spectrophotometric method has been described for the determination of thiamine. The method is based on the precipitation of sulphur as barium sulphate using barium chloride after oxidation of thiamine by sodium hydroxide and hydrogen peroxide. Beer's law was obeyed over the concentration range from 2-32 µg/mL thiamine. The average of standard deviation was 1.18×10-2; standard error, 5.20×10-3; relative standard deviation, 0.51%; relative standard error, 5.40×10-3; 95% coefficient, 1.48×10-2; the limit of detection, 0.41 µg/mL; the limit of quantification, 1.38 µg/mL and recovery, 100.03% thiamine. Finally, the developed method was applied to the determination of thiamine in pharmaceutical formulations.


Keywords


Thiamine; Oxidation; Precipitation; Determination; Barium sulphate; Spectrophotometric

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DOI: 10.5155/eurjchem.5.1.81-84.881

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Citations

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DOI: 10.1007/s00289-016-1891-0
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[2]. Shadpour Mallakpour, Soheila Mansourzadeh
Application of CuO nanoparticles modified with vitamin B1 for the production of poly(vinyl alcohol)/CuO nanocomposite films with enhanced optical, thermal and mechanical properties
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DOI: 10.1002/pat.4068
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[3]. Katie A. Edwards, Nicole Tu-Maung, Krystal Cheng, Binbin Wang, Antje J. Baeumner, Clifford E. Kraft
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[4]. Hui Zhang, Hongyun Chen, Hongxi Li, Shuang Pan, Yalin Ran, Xiaoli Hu
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How to cite


Al-Ahmary, K. Eur. J. Chem. 2014, 5(1), 81-84. doi:10.5155/eurjchem.5.1.81-84.881
Al-Ahmary, K. A simple spectrophotometric method for determination of thiamine (vitamin B1) in pharmaceuticals. Eur. J. Chem. 2014, 5(1), 81-84. doi:10.5155/eurjchem.5.1.81-84.881
Al-Ahmary, K. (2014). A simple spectrophotometric method for determination of thiamine (vitamin B1) in pharmaceuticals. European Journal of Chemistry, 5(1), 81-84. doi:10.5155/eurjchem.5.1.81-84.881
Al-Ahmary, Khairia. "A simple spectrophotometric method for determination of thiamine (vitamin B1) in pharmaceuticals." European Journal of Chemistry [Online], 5.1 (2014): 81-84. Web. 14 Nov. 2019
Al-Ahmary, Khairia. "A simple spectrophotometric method for determination of thiamine (vitamin B1) in pharmaceuticals" European Journal of Chemistry [Online], Volume 5 Number 1 (31 March 2014)

DOI Link: https://doi.org/10.5155/eurjchem.5.1.81-84.881

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