European Journal of Chemistry

Composition and content of total phenolics and flavonoids, and antioxidant activity of Trigonella isthmocarpa

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Published: Mar 31, 2013
DOI: https://doi.org/10.5155/eurjchem.4.1.7-9.719
Keywords:
HPLC, Fabaceae, Total phenol, T. isthmocarpa, Total flavonoid, DPPH radical scavenging activity
Section
Research Article
Issue
Vol. 4 No. 1 (2013): March 2013
Dates
Received 2012-12-21
Accepted 2013-01-07
Published 2013-03-31


Main Article Content

Serife Selma Uras Gungor
Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenişehir Campus, 33169 Mersin, Turkey
Sevda Guzel Ozay
Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenişehir Campus, 33169 Mersin, Turkey
Ahmet Ilcim
Department of Biology, Faculty of Arts and Science, Mustafa Kemal University, 31000, Antakya, Hatay, Turkey
Gamze Kokdil
Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenişehir Campus, 33169 Mersin, Turkey

Abstract

The aqueous methanolic extracts obtained from aerial parts and seeds of T. isthmocarpa Boiss. & Bal. from Turkey were analyzed by reversed-phase HPLC for their phenolic acid and flavonoid composition. Total phenolic content was determined using the Folin-Ciocalteu assay, while total flavonoid content was measured spectrometrically with AlCl3 assay. Primary antioxidant activity in terms of free radical scavenging activities of both extracts was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest total flavonoid and total phenolic content was determined in the extract of aerial parts (830.8 mg/100 g and 877.9 mg gallic acid equivalent/100 g, respectively). The extracts of aerial parts and seeds had the high antioxidant activity (87.2 % and 78.2%, respectively). Gallic acid, caffeic acid, luteolin, apigenin and kaempferol were detected as phenolic compounds in both extracts by HPLC methods. In addition, an isoflavone, formononetin was determined in the extract from aerial parts of the plant. The present results suggest that T. isthmocarpa have shown potential a source of natural antioxidants.

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Gungor, S. S. U.; Ozay, S. G.; Ilcim, A.; Kokdil, G. Composition and Content of Total Phenolics and Flavonoids, and Antioxidant Activity of Trigonella Isthmocarpa. Eur. J. Chem. 2013, 4, 7-9.

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