European Journal of Chemistry 2014, 5(3), 545-549 | doi: https://doi.org/10.5155/eurjchem.5.3.545-549.1066 | Get rights and content






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Validated RP-HPLC and HPTLC methods for simultaneous estimation of febuxostat and diclofenac sodium in pharmaceutical dosage form


Ponnuveetil Gopi Sunitha (1) , Kaliappan Ilango (2,*)

(1) Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai, 600003, Tamil Nadu, India
(2) Department of Pharmaceutical Chemistry, Sri Ramaswamy Memorial College of Pharmacy, Sri Ramaswamy Memorial University, Kattankulathur, 603203, Tamil Nadu, India
(*) Corresponding Author

Received: 05 Apr 2014 | Revised: 01 May 2014 | Accepted: 01 May 2014 | Published: 30 Sep 2014 | Issue Date: September 2014

Abstract


The present work reports a reverse phase high performance liquid chromatography (RP-HPLC) method and high performance thin layer chromatography (HPTLC) method for the simultaneous determination of febuxostat and diclofenac sodium in pharmaceutical dosage form. HPLC was performed using a Thermo Hypersil C18 column (250 × 4.6 mm i.d., 5 μm particle size) using a mixture of methanol: KH2PO4 (0.02 M) (70:30, v:v) as mobile phase. Ultraviolet detection was carried out at 280 nm. The retention time of febuxostat and diclofenac sodium were found to be 6.725 and 8.892 min, respectively. The HPTLC separation was conducted on Merck HPTLC aluminum sheets of silica gel 60 F254 as stationary phase using toluene:methanol in the ratio of 7:3 (v:v) as the mobile phase. The detection of febuxostat and diclofenac sodium was carried out at 297 nm. The Rf values of febuxostat and diclofenac sodium were found to be 0.48 and 0.60, respectively. Both the methods were validated as per ICH guidelines. The proposed methods were found to be suitable for the quantification of the selected combination of drugs in pharmaceutical dosage form.


Keywords


Tablet; HPTLC; RP-HPLC; Validation; Diclofenac; Febuxostat

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DOI: 10.5155/eurjchem.5.3.545-549.1066

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Funding information


Centaur Pharmaceuticals Ltd., Pune, India

Citations

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[1]. Fawzi A. El-Yazbi, Omayma A. Amin, Eman I. El-Kimary, Essam F. Khamis, Sameh E. Younis
HPTLC and Spectrophotometric Estimation of Febuxostat and Diclofenac Potassium in Their Combined Tablets
Journal of Chromatographic Science  54(7), 1146, 2016
DOI: 10.1093/chromsci/bmw091
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[2]. Abdel-Maaboud I. Mohamed, Mahmoud A. Omar, Sayed M. Derayea, Mohamed A. Hammad, Abobakr A. Mohamed
Innovative thin-layer chromatographic method combined with fluorescence detection for specific determination of Febuxostat: Application in biological fluids
Talanta  176, 318, 2018
DOI: 10.1016/j.talanta.2017.08.051
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[3]. Mahmoud A. Omar, Abdel-Maaboud I. Mohamed, Sayed M. Derayea, Mohamed A. Hammad, Abobakr A. Mohamed
Specific and highly sensitive spectrofluorimetric method for determination of febuxostat in its tablets and real human plasma. Application to stability studies
RSC Advances  6(77), 73432, 2016
DOI: 10.1039/C6RA11246C
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[4]. Fawzy A El-Yazbi, Omayma A Amin, Eman I El-Kimary, Essam F Khamis, Sameh E Younis, Mohammed AW Elkhatib, Ahmed F El-Yazbi
A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: pharmacokinetic outcomes
Bioanalysis  11(1), 41, 2019
DOI: 10.4155/bio-2018-0219
/


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How to cite


Sunitha, P.; Ilango, K. Eur. J. Chem. 2014, 5(3), 545-549. doi:10.5155/eurjchem.5.3.545-549.1066
Sunitha, P.; Ilango, K. Validated RP-HPLC and HPTLC methods for simultaneous estimation of febuxostat and diclofenac sodium in pharmaceutical dosage form. Eur. J. Chem. 2014, 5(3), 545-549. doi:10.5155/eurjchem.5.3.545-549.1066
Sunitha, P., & Ilango, K. (2014). Validated RP-HPLC and HPTLC methods for simultaneous estimation of febuxostat and diclofenac sodium in pharmaceutical dosage form. European Journal of Chemistry, 5(3), 545-549. doi:10.5155/eurjchem.5.3.545-549.1066
Sunitha, Ponnuveetil, & Kaliappan Ilango. "Validated RP-HPLC and HPTLC methods for simultaneous estimation of febuxostat and diclofenac sodium in pharmaceutical dosage form." European Journal of Chemistry [Online], 5.3 (2014): 545-549. Web. 2 Jun. 2020
Sunitha, Ponnuveetil, AND Ilango, Kaliappan. "Validated RP-HPLC and HPTLC methods for simultaneous estimation of febuxostat and diclofenac sodium in pharmaceutical dosage form" European Journal of Chemistry [Online], Volume 5 Number 3 (30 September 2014)

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DOI Link: https://doi.org/10.5155/eurjchem.5.3.545-549.1066

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