European Journal of Chemistry 2011, 2(4), 519-523 | doi: https://doi.org/10.5155/eurjchem.2.4.519-523.483 | Get rights and content






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Statistical analysis in enrichment of total whey protein by continuous foam fractionation method


Goutam Mukhopadhyay (1,*) , Jasmina Khanam (2) , Arunabha Nanda (3) , Nripendra Nath Bala (4) , Won Chun Oh (5)

(1) Bengal Chemists and Druggists Association, College of Pharmacy and Technology, Kolkata-127, 700127, India
(2) Department of Pharmaceutical Technology, Jadavpur University, Kolkata-32, 700032, India
(3) Department of Pharmaceutical Technology, Jadavpur University, Kolkata-32, 700032, India
(4) Bengal Chemists and Druggists Association, College of Pharmacy and Technology, Kolkata-127, 700127, India
(5) Department of Advance Material Science and Engineering, Hanseo University, Seosansi, Chungnam-356-706, Korea
(*) Corresponding Author

Received: 05 Jul 2011 | Revised: 01 Sep 2011 | Accepted: 14 Aug 2011 | Published: 31 Dec 2011 | Issue Date: December 2011

Abstract


The objective of the present study was to optimize the operating conditions in the separation of the total whey proteins from whey by continuous foam fractionation method using response surface methodology (RSM). The effects of the different process variables such as pH (X1) of proteins in feed, gas flow rate, (X2) of initial feed solution, protein: surfactant ratio (X3) and volumetric flow rate (X4) where investigated on the performance criteria of fractionation of raw processed whey. Four factors, three levels Box-Behnken design was used for the optimization procedure. Quadratic model regression equations and response surface plots correlate independent variables (X1, X2, X3 and X4) and dependent variables (response) such as concentration of Foamate (Cf), Enrichment ratio (Er), and percentage Recovery (% Rp) of total whey proteins. All the four factors had significant effects on the response variables. The model predicted that the optimized values of the factors (X1, X2, X3 and X4) such as 5, 290, 1.5 and 14, respectively. The predicted responses were (concentration of foamate, enrichment ratio, and percentage recovery) such as 6647.32, 13.27, and 78.02, respectively. Experiments were performed with the predicted values of factors.

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Keywords


Box-Behnken design; Optimization; Foam separation; Whey; Enrichment ratio; Separation ration

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DOI: 10.5155/eurjchem.2.4.519-523.483

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Funding information


Bengal Chemists and Druggists Association College of Pharmacy and Technology, Kolkata, India; Jadavpur University, Kolkata, India and Hanseo University, Korea

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How to cite


Mukhopadhyay, G.; Khanam, J.; Nanda, A.; Bala, N.; Oh, W. Eur. J. Chem. 2011, 2(4), 519-523. doi:10.5155/eurjchem.2.4.519-523.483
Mukhopadhyay, G.; Khanam, J.; Nanda, A.; Bala, N.; Oh, W. Statistical analysis in enrichment of total whey protein by continuous foam fractionation method. Eur. J. Chem. 2011, 2(4), 519-523. doi:10.5155/eurjchem.2.4.519-523.483
Mukhopadhyay, G., Khanam, J., Nanda, A., Bala, N., & Oh, W. (2011). Statistical analysis in enrichment of total whey protein by continuous foam fractionation method. European Journal of Chemistry, 2(4), 519-523. doi:10.5155/eurjchem.2.4.519-523.483
Mukhopadhyay, Goutam, Jasmina Khanam, Arunabha Nanda, Nripendra Nath Bala, & Won Chun Oh. "Statistical analysis in enrichment of total whey protein by continuous foam fractionation method." European Journal of Chemistry [Online], 2.4 (2011): 519-523. Web. 11 Aug. 2020
Mukhopadhyay, Goutam, Khanam, Jasmina, Nanda, Arunabha, Bala, Nripendra, AND Oh, Won. "Statistical analysis in enrichment of total whey protein by continuous foam fractionation method" European Journal of Chemistry [Online], Volume 2 Number 4 (31 December 2011)

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DOI Link: https://doi.org/10.5155/eurjchem.2.4.519-523.483

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