European Journal of Chemistry 2014, 5(2), 219-226. doi:10.5155/eurjchem.5.2.219-226.966
OPEN ACCESS

Development and validation of stability indicating spectrophotometric and HPTLC methods for determination of acemetacin


Ibrahim Ahmed Naguib (1) , Eglal Abdelhamid Abdelaleem (2) , Hala Elsayed Zaazaa (3) , Essraa Abd ElWahab Hussein (4,*)

(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, 62514, Beni-Suef, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, 62514, Beni-Suef, Egypt
(3) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini, 11562 Cairo, Egypt
(4) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, 62514, Beni-Suef, Egypt
(*) Corresponding Author

Received: 09 Nov 2013, Accepted: 05 Dec 2013, Published: 30 Jun 2014

Abstract


Three simple and sensitive methods were developed for the determination of acemetacin (ACM) in presence of its degradation product, indomethacin (DEG). Several methods were evaluated and are presented. Method A was based on measuring the peak amplitude of the first derivative of the ratio spectra 1DD at 244 nm. In method B, mean centering of ratio spectra method (MCR) was applied, which depends on measuring the mean centered values of ratio spectra of ACM at 234 nm. Method C provided separation of ACM from its DEG on pre-activated silica gel 60F254 HPTLC plates using hexane:ethyl acetate:glacial acetic acid (6:4:0.3, v:v:v) as developing system followed by scanning at 254 nm. The suggested methods were validated in compliance with the International Conference on Harmonisation (ICH) guidelines and were successfully applied for quantification of ACM in its commercial capsule. The proposed methods were also statistically compared to a reported HPLC method with no significant difference in performance; indicating the ability of the proposed method to be reliable and suitable for routine analysis of drug product.


Keywords


HPTLC; Validation; Acemetacin; Degradation; First derivative of ratio spectra; Mean centering of ratio spectra

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DOI: 10.5155/eurjchem.5.2.219-226.966

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[1]. Maha Mahmoud Abou-El Alamin
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[8]. Mohamed A. Abdelgawad, Asmaa I. Owis, Nada S. Abdelwahab
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How to cite


Naguib, I.; Abdelaleem, E.; Zaazaa, H.; Hussein, E. Eur. J. Chem. 2014, 5(2), 219-226. doi:10.5155/eurjchem.5.2.219-226.966
Naguib, I.; Abdelaleem, E.; Zaazaa, H.; Hussein, E. Development and validation of stability indicating spectrophotometric and HPTLC methods for determination of acemetacin. Eur. J. Chem. 2014, 5(2), 219-226. doi:10.5155/eurjchem.5.2.219-226.966
Naguib, I., Abdelaleem, E., Zaazaa, H., & Hussein, E. (2014). Development and validation of stability indicating spectrophotometric and HPTLC methods for determination of acemetacin. European Journal of Chemistry, 5(2), 219-226. doi:10.5155/eurjchem.5.2.219-226.966
Naguib, Ibrahim, Eglal Abdelhamid Abdelaleem, Hala Elsayed Zaazaa, & Essraa Abd ElWahab Hussein. "Development and validation of stability indicating spectrophotometric and HPTLC methods for determination of acemetacin." European Journal of Chemistry [Online], 5.2 (2014): 219-226. Web. 7 Dec. 2019
Naguib, Ibrahim, Abdelaleem, Eglal, Zaazaa, Hala, AND Hussein, Essraa. "Development and validation of stability indicating spectrophotometric and HPTLC methods for determination of acemetacin" European Journal of Chemistry [Online], Volume 5 Number 2 (30 June 2014)

DOI Link: https://doi.org/10.5155/eurjchem.5.2.219-226.966

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