European Journal of Chemistry

Derivative spectrophotometric method for simultaneous determination of ezetimibe and simvastatin in combined tablets

Main Article Content

Bahia Abbas Moussa
Marwa Fadel Mohamed
Nadia Fayek Youssef


The introduced study portrays the development and validation of an effortless, speedy and selective first order derivative zero-crossing spectrophotometric method to estimate ezetimibe and simvastatin simultaneously in combined drug products over and above in the presence of ezetimibe alkaline degradation products. Ezetimibe and its alkaline degradates spectra showed zero-crossing point at 249 nm, so the amplitude at 249 nm could be used for calculating the concentration of simvastatin. While simvastatin and ezetimibe alkaline degradates spectra showed a zero-reading value at 261 nm, so ezetimibe could be determined by measuring the amplitude at 261 nm. Regression plots revealed good linear relationships in the concentration range 1-16 µg/mL with accuracy checked by conducting recovery studies; average recovery was 99.61 ± 1.183 and 100.47 ± 1.310 for ezetimibe and simvastatin, respectively. The suggested first derivative spectrophotometric method was successfully applied for the determination of the cited drugs in "Inegy 10/10, 10/20 and 10/40 tablets". Satisfactory results were obtained for the recovery of both drugs and were in good agreement with the labeled amounts.  Method validation was estimated according to USP guidelines.


icon graph This Abstract was viewed 2353 times | icon graph Article PDF downloaded 843 times

How to Cite
Moussa, B. A.; Mohamed, M. F.; Youssef, N. F. Derivative Spectrophotometric Method for Simultaneous Determination of Ezetimibe and Simvastatin in Combined Tablets. Eur. J. Chem. 2010, 1, 348-351.

Article Details

Crossref - Scopus - Google - European PMC

[1]. Sweetman, S. C. Martindale, The Complete Drug Reference, 36th Edition, The Pharmaceutical Press, London, 2009.

[2]. Brunton, L. L.; Lazo, J. S.; Parker, K. L. Goodman and Gilman's, The Pharmacological Basis of Therapeutics. McGraw-Hill Book Co., New York, NY, 2006.

[3]. Sonawane, S. S.; Shirkhedkar, A. A.; Fursule, R.A.; Surana, S. J. Eurasian J. Anal. Chem. 2006, 1, 31-41.

[4]. Erk, N. Pharmazie 2002, 57, 817-819.

[5]. Dhaneshwar, S. S.; Dhaneshwar, S. R.; Deshpande, P.; Patil, M. Acta Chromatogr. 2007, 19, 141-148.

[6]. Dixit, R. P.; Barhate, C. R.; Nagarsenker, M. S. Chromatographia 2008, 67, 101-107.

[7]. Komsta, L.; Skibinski, R.; Hopkala, H.; Winiarcyzk-Serwacka, M. Chem. Anal. (Warsaw, Pol.) 2007, 52, 771-779.

[8]. Sistla, R.; Tata, V. S. S. K.; Kashyap, Y. V.; Chandrasekar, D.; Diwan, P.V. J. Pharm. Biomed. Anal. 2005, 39, 517-522.

[9]. Li, S. J.; Liu, G.Y.; Jia, J.Y.; Li, X.C.; Yu, C. J. Pharm. Biomed. Anal. 2006, 40, 987-992.

[10]. Oliveria, P. R.; Brum, L. Jr.; Fronza, M.; Bernard, L. S.; Masiero, S. M. K.; Dalmora, S. L. Chromatographia 2006, 63, 315-320.

[11]. Singh, S.; Singh, B.; Bahuguna, R.; Wadhwa, L.; Saxena, R. J. Pharm. Biomed. Anal. 2006, 41, 1037-1040.

[12]. Oswald, S.; Scheuch, E.; Cascorbi, I.; Siegmund, W. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2006, 830,143-150.

[13]. Ashfaq M.; Ullahkhan I.; Qutab S. S.; Naeemrazzaq S. J. Chil. Chem. Soc. 2007, 52, 1220-1223.

[14]. Basha, S. J. S.; Naveed, S. A.; Tiwari, N. K.; Shashikumar, D.; Muzeeb, S.; Kumar, T. R.; Kumar, N.V.; Rao, N. P.; Srinivas, N.; Mullangi, R.; Srinivas, N. R. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2007, 853, 88-96.

[15]. Ozaltin, N.; Ucakturk, E. Chromatographia 2007, 66, S87-S91.

[16]. Doshi, A. S.; Kachhadia, P. K.; Joshi, H. S. Chromatographia 2008, 67, 137-142.

[17]. Seshachalam, U.; Kothapally, C. B. J. Liq. Chromatogr. Relat. Technol. 2008, 31, 714-721.

[18]. Iwabuchi, H.; Kitazawa, E.; Kobayashi, N.; Watanabe, H.; Kanai, M.; Nakamura, K. Biol. Mass Spectrom. 1994, 23, 540-546.

[19]. Ochiai, H.; Uchiyama, N.; Imagaki, K.; Hata, S.; Kamei, T. J. chromatogr. B: Biomed. Appl. 1997, 694, 211-217.

[20]. Jemal, M.; Ouyang, Z.; Powell, M. L. J. Pharm. Biomed. Anal. 2000, 23, 323-340.

[21]. Zhao, J. J.; Xie, I. H.; Yang, A. Y.; Roadcap, B. A.; Rogers, J. D. J. Mass Spectrom. 2000, 35, 1133-1143.

[22]. Zhang, N. Y.; Yang, A.; Rogers, J. D.; Zhao, J. J. J. Pharm. Biomed. Anal. 2004, 34, 175-287.

[23]. Yang, A. Y.; Sun, L.; Musson, D. G.; Zhao, J. J. J. Pharm. Biomed. Anal. 2005, 38, 521-527.

[24]. Barrett, B.; Huclova, J.; Borek-Dohalsky, V.; Nemec, B.; Jelinek, I. J. Pharm. Biomed. Anal. 2006, 41, 517-526.

[25]. Malenovic, A.; Medenica, M.; Ivanovic, D.; Jancic, B. Chromatographia 2006, 63, S95-S100.

[26]. Senthamil S. P.; Pal, T. K. J. Pharm. Biomed. Anal. 2009, 49, 780-785.

[27]. Dalmora, S. L.; Oliveria, P. R.; Barth, T.; Todeschini, V. J. Anal. Sci. 2008, 24, 499-503.

[28]. Srinivasu, M. K.; Raju, A. N.; Reddy, G. O. J. Pharm. Biomed. Anal. 2002, 29, 715-721.

[29]. Morris, M. J.; Gilbert, J. D.; Hsieh, J. Y.-K.; Matuszewski, B. K.; Ramjit, H. G.; Bayne, W. F. Biol. Mass Spectrom. 1993, 22, 1-8.

[30]. Raw Material In House Specifications; Glenmark Pharmaceuticals, Mumbai, India, 2003.

[31]. The United States Pharmacopoeia (USP 32) and National Formulary (NF 27); Asian Ed., 2009.

[32]. Morelli, B. J. Pharm. Sci. 1988, 77, 615-621.

[33]. Morelli, B. J. Pharm. Sci. 1988, 77, 1042-1045.

[34]. Morelli, B. J. Pharm. Sci. 1995, 84, 34-37.

[35]. Talsky, G.; Marying, L.; Kreuzer, H. Angew. Chem. Int. Ed. Engl. 1978, 17, 785-799.

[36]. OHaver, T.C. Anal. Chem. 1979, 51, 91A-100A.

[37]. Rojas, F.S.; Ojeda, C.B.; Pavon, J. M.C. Talanta 1988, 35, 753-761.


Supporting Agencies

Most read articles by the same author(s)

Dimensions - Altmetric - scite_ - PlumX

Downloads and views


Download data is not yet available.


Metrics Loading ...
License Terms

License Terms


Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License ( By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License ( are administered by Atlanta Publishing House LLC (European Journal of Chemistry).