European Journal of Chemistry

Two spectrophotometric methods for the determination of azithromycin and roxithromycin in pharmaceutical preparations

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Published: Sep 30, 2017
DOI: https://doi.org/10.5155/eurjchem.8.3.203-210.1574
Keywords:
Macrolides, Azithromycin, Metal complex, Roxithromycin, Spectrophotometry, N-Bromosuccinimide
Section
Research Article
Issue
Vol. 8 No. 3 (2017): September 2017
Dates
Received 2017-04-19
Accepted 2017-05-30
Published 2017-09-30


Main Article Content

Fawzia Ahmed Ibrahim
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
Mary Elias Kamel Wahba
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
Galal Magdy Galal
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, 35712, Egypt

Abstract

Two new and simple spectrophotometric procedures have been proposed and validated for estimation of two important macrolide antibiotics namely, azithromycin dihydrate and roxithromycin. Method I depends on complex formation between any of the two drugs and copper in acidic medium where the absorbances of the produced complexes are measured at 250 and 264 nm with linearity ranges of 1.0-100.0 and 2.0-130.0 µg/mL for the two drugs, respectively. Method II depends on the reaction of these drugs with N-bromosuccinimide forming a product which is yellow colored, measured at 264 and 278 nm, with linearity ranges of 2.0-140.0 and 3.0-160.0 µg/mL for azithromycin dihydrate and roxithromycin, respectively. The proposed methods were subjected to detailed validation procedure; moreover they were used for the estimation of the concerned drugs in their different dosage forms. Study of the reactions stoichiometry was carried out; furthermore, a reaction mechanism proposal was presented.


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Ibrahim, F. A.; Wahba, M. E. K.; Galal, G. M. Two Spectrophotometric Methods for the Determination of Azithromycin and Roxithromycin in Pharmaceutical Preparations. Eur. J. Chem. 2017, 8, 203-210.

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