European Journal of Chemistry 2021, 12(3), 299-303 | doi: https://doi.org/10.5155/eurjchem.12.3.299-303.2072 | Get rights and content

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Crystallization of 3-hexulose-6-phosphate synthase


Masoud Delfi (1) orcid , Leila Mahdavian (2,*) orcid , Mohammad Sattarifar (3) orcid , Nina Hakulinen (4) orcid , Juha Rouvinen (5) orcid

(1) Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland
(2) Department of Chemistry, Doroud Branch, Islamic Azad University, P.O. Box: 133. Doroud. Iran
(3) Department of Chemistry, Doroud Branch, Islamic Azad University, P.O. Box: 133. Doroud. Iran
(4) Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland
(5) Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland
(*) Corresponding Author

Received: 18 Jan 2021 | Revised: 18 Jun 2021 | Accepted: 15 Jul 2021 | Published: 30 Sep 2021 | Issue Date: September 2021

Abstract


The crystal structures can reveal detailed information about the overall structure, active site structure, and functional mechanism of enzymes. This study focused on the crystallization of 3-hexulose-6-phosphate synthase from Methylomonas aminofaciens 77a, to produce higher resolution crystals for precise structural characterization. 3-Hexulose-6-phosphate synthase is from Methylomonas aminofaciens 77a (EC 4.1.2.43). It belongs to the orotidine 5'-monophosphate decarboxylase superfamily, and acts as a key enzyme for a ribulose-monophosphate cycle of formaldehyde fixation and detoxification. 3-Hexulose-6-phosphate synthase catalyzes the aldol condensation of formaldehyde with D-ribulose-5-phosphate. For the maximum activity, 3-hexulose-6-phosphate synthase requires Mg2+ or Mn2+ as ligands. MaHPS crystallized at the concentration of 7 mg/mL and conditions consisting of 0.2 M MgCl2, 18% PEG 3350 at pH = 7.0.


Keywords


Protein crystallization; Formaldehyde fixation; Methylotrophic bacteria; Methylomonas aminofaciens 77a; 3-Hexulose-6-phosphate synthase; Ribulose monophosphate pathway

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DOI: 10.5155/eurjchem.12.3.299-303.2072

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Funding information


Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland.

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How to cite


Delfi, M.; Mahdavian, L.; Sattarifar, M.; Hakulinen, N.; Rouvinen, J. Eur. J. Chem. 2021, 12(3), 299-303. doi:10.5155/eurjchem.12.3.299-303.2072
Delfi, M.; Mahdavian, L.; Sattarifar, M.; Hakulinen, N.; Rouvinen, J. Crystallization of 3-hexulose-6-phosphate synthase. Eur. J. Chem. 2021, 12(3), 299-303. doi:10.5155/eurjchem.12.3.299-303.2072
Delfi, M., Mahdavian, L., Sattarifar, M., Hakulinen, N., & Rouvinen, J. (2021). Crystallization of 3-hexulose-6-phosphate synthase. European Journal of Chemistry, 12(3), 299-303. doi:10.5155/eurjchem.12.3.299-303.2072
Delfi, Masoud, Leila Mahdavian, Mohammad Sattarifar, Nina Hakulinen, & Juha Rouvinen. "Crystallization of 3-hexulose-6-phosphate synthase." European Journal of Chemistry [Online], 12.3 (2021): 299-303. Web. 20 Oct. 2021
Delfi, Masoud, Mahdavian, Leila, Sattarifar, Mohammad, Hakulinen, Nina, AND Rouvinen, Juha. "Crystallization of 3-hexulose-6-phosphate synthase" European Journal of Chemistry [Online], Volume 12 Number 3 (30 September 2021)

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