European Journal of Chemistry

Crystallization of 3-hexulose-6-phosphate synthase

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Published: Sep 30, 2021
DOI: https://doi.org/10.5155/eurjchem.12.3.299-303.2072
Keywords:
Protein crystallization, Formaldehyde fixation, Methylotrophic bacteria, Methylomonas aminofaciens 77a, 3-Hexulose-6-phosphate synthase, Ribulose monophosphate pathway
Section
Research Article
Issue
Vol. 12 No. 3 (2021): September 2021
Dates
Received 2021-01-18
Accepted 2021-07-15
Published 2021-09-30
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Masoud Delfi
Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland
https://orcid.org/0000-0001-5003-1241
Leila Mahdavian
Department of Chemistry, Doroud Branch, Islamic Azad University, P.O. Box: 133. Doroud. Iran
https://orcid.org/0000-0001-9582-4667
Mohammad Sattarifar
Department of Chemistry, Doroud Branch, Islamic Azad University, P.O. Box: 133. Doroud. Iran
https://orcid.org/0000-0002-3788-8838
Nina Hakulinen
Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland
https://orcid.org/0000-0003-4471-7188
Juha Rouvinen
Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland
https://orcid.org/0000-0003-1843-5718

Abstract

The crystal structures can reveal detailed information about the overall structure, active site structure, and functional mechanism of enzymes. This study focused on the crystallization of 3-hexulose-6-phosphate synthase from Methylomonas aminofaciens 77a, to produce higher resolution crystals for precise structural characterization. 3-Hexulose-6-phosphate synthase is from Methylomonas aminofaciens 77a (EC 4.1.2.43). It belongs to the orotidine 5'-monophosphate decarboxylase superfamily, and acts as a key enzyme for a ribulose-monophosphate cycle of formaldehyde fixation and detoxification. 3-Hexulose-6-phosphate synthase catalyzes the aldol condensation of formaldehyde with D-ribulose-5-phosphate. For the maximum activity, 3-hexulose-6-phosphate synthase requires Mg2+ or Mn2+ as ligands. MaHPS crystallized at the concentration of 7 mg/mL and conditions consisting of 0.2 M MgCl2, 18% PEG 3350 at pH = 7.0.


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(1)
Delfi, M.; Mahdavian, L.; Sattarifar, M.; Hakulinen, N.; Rouvinen, J. Crystallization of 3-Hexulose-6-Phosphate Synthase. Eur. J. Chem. 2021, 12, 299-303.

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Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland.
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