

Application of two different spectrophotometric approaches for the determination of a new antihypertensive combination: Graphical and statistical representation of the data
Ragaa Magdy (1)




(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th October, 12566, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th October, 12566, Egypt
(3) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(4) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(*) Corresponding Author
Received: 10 Mar 2022 | Revised: 06 May 2022 | Accepted: 08 May 2022 | Published: 30 Sep 2022 | Issue Date: September 2022
Abstract
Specific, and precise spectrophotometric methods are developed and validated for the simultaneous determination of the binary antihypertensive mixture nebivolol hydrochloride and valsartan in the zero-order spectrum. The methods applied for the determination of this antihypertensive mixture are constant center spectrophotometric resolution technique, constant center spectrum subtraction resolution technique, and advanced concentration value. Nebivolol hydrochloride was determined by its zero order spectra at 280 and 213 nm while for valsartan it determined by its zero order spectra at 250 nm. All developed methods were applied for the determination of the cited drugs in the pharmaceutical formulation and the results obtained were statistically compared with each other and with those of the reported method. The comparison showed that there is no significant difference between the proposed methods and the reported method regarding both accuracy and precision.
Announcements
Our editors have decided to support scientists to publish their manuscripts in European Journal of Chemistry without any financial constraints.
1- The article processing fee will not be charged from the articles containing the single-crystal structure characterization or a DFT study between September 15, 2023 and October 31, 2023 (Voucher code: FALL2023).
2. A 50% discount will be applied to the article processing fee for submissions made between September 15, 2023 and October 31, 2023 by authors who have at least one publication in the European Journal of Chemistry (Voucher code: AUTHOR-3-2023).
3. Young writers will not be charged for the article processing fee between September 15, 2023 and October 31, 2023 (Voucher code: YOUNG2023).
Editor-in-Chief
European Journal of Chemistry
Keywords
Full Text:
PDF

DOI: 10.5155/eurjchem.13.3.267-272.2272
Links for Article
| | | | | | |
| | | | | | |
| | | |
Related Articles
Article Metrics


References
[1]. Bakris, G. L.; Hart, P.; Ritz, E. Beta blockers in the management of chronic kidney disease. Kidney Int. 2006, 70, 1905-1913.
https://doi.org/10.1038/sj.ki.5001835
[2]. Denker, M. G.; Cohen, D. L. What is an appropriate blood pressure goal for the elderly: review of recent studies and practical recom-mendations. Clin. Interv. Aging 2013, 8, 1505-1517.
https://doi.org/10.2147/CIA.S33087
[3]. Passino, C.; Sciarrone, P.; Vergaro, G.; Borrelli, C.; Spiesshoefer, J.; Gentile, F.; Emdin, M.; Giannoni, A. Sacubitril-valsartan treatment is associated with decrease in central apneas in patients with heart failure with reduced ejection fraction. Int. J. Cardiol. 2021, 330, 112-119.
https://doi.org/10.1016/j.ijcard.2021.02.012
[4]. Hemdan, A.; Magdy, R.; Farouk, M. Response surface design as a powerful tool for the development of environmentally benign HPLC methods for the determination of two antihypertensive combinations: Greenness assessment by two green analytical chemistry evaluation tools. J. Sep. Sci. 2018, 41, 3213-3223.
https://doi.org/10.1002/jssc.201800317
[5]. Farrell, B.; French Merkley, V.; Ingar, N. Reducing pill burden and helping with medication awareness to improve adherence. Can. Pharm. J. (Ott.) 2013, 146, 262-269.
https://doi.org/10.1177/1715163513500208
[6]. Sander, G. E.; Giles, T. D. Nebivolol and valsartan as a fixed-dose combination for the treatment of hypertension. Expert Opin. Pharmacother. 2015, 16, 763-770.
https://doi.org/10.1517/14656566.2015.1020790
[7]. Gupta, S.; Wright, H. M. Nebivolol: a highly selective beta1-adrenergic receptor blocker that causes vasodilation by increasing nitric oxide. Cardiovasc. Ther. 2008, 26, 189-202.
https://doi.org/10.1111/j.1755-5922.2008.00054.x
[8]. Koumaras, C.; Tziomalos, K.; Stavrinou, E.; Katsiki, N.; Athyros, V. G.; Mikhailidis, D. P.; Karagiannis, A. Effects of renin-angiotensin-aldosterone system inhibitors and beta-blockers on markers of arterial stiffness. J. Am. Soc. Hypertens. 2014, 8, 74-82.
https://doi.org/10.1016/j.jash.2013.09.001
[9]. Rajoriya, V.; Kashaw, V. RP-HPLC method for the simultaneous estimation of nebivolol hydrochloride and valsartan. Anal. Chem. Lett. 2017, 7, 520-530.
https://doi.org/10.1080/22297928.2017.1362994
[10]. Prashanthi, D.; Ramesh, B.; Jayashree, V. Simultaneous estimation of nebivolol and valsartan by RP-HPLC method in tablet dosage form. Int. J. Chem. Pharm. Sci. 2018, 9 (2), 6-8. http://www.ijcps.com/files/vol9issue2/2.pdf (accessed May 5, 2022).
[11]. Ramachandran, S.; Mandal, B. K.; Navalgund, S. G. Stability-indicating HPLC method for the simultaneous determination of valsartan and ezetimibe in pharmaceuticals. Trop. J. Pharm. Res. 2014, 13, 809-817.
https://doi.org/10.4314/tjpr.v13i5.23
[12]. Kumar, R. A.; Babu, G. R.; Sowjanya; Ramayyappa Validated RP-HPLC method for the estimation of nebivolol hydrochloride and valsartan in combined tablet dosage form. Asian J. Res. Chem. 2021, 168-172.
https://doi.org/10.52711/0974-4150.2021.00031
[13]. Annadi, A.; Shoheib, R.; Mohamed, A. Assessments of Valsartan in the presence of Nebivolol or Amlodipine in solid formulations and its discriminative dissolution behavior via RP‑HPLC and RP‑UPLC methods. Egypt. J. Chem. 2020, 63 (8), 8-9.
https://doi.org/10.21608/ejchem.2020.18176.2116
[14]. Satriani, E.; Desni, R.; Rivai, H. Review of valsartan analysis methods from 2000 to 2020. World J. Pharm. Pharmaceuti. Sci. 2022, 9 (7), 1268-1287. https://storage.googleapis.com/journal-uploads/wjpps/article_issue/1593511035.pdf (accessed May 5, 2022).
[15]. Vyas, A.; Nathwani, G.; Patel, A.; Patel, A.; Patel, N.; Makvana, N. Stability indicating assay method for simultaneous estimation of nebivolol and valsartan in pharmaceutical dosage form by RP-HPLC. Asian J. Green Chem. 2018, 2, 227-245.
[16]. Gole, R.; Monahar, K. R. Method development and validation for the simultaneous estimation of Nebivolol and Valsartan in bulk and solid dosage form by RP-HPLC. IOSR J. Pharm. Bio. Sci. (IOSR-JPBS) 2020, 15 (1), 63-69. https://www.iosrjournals.org/iosr-jpbs/papers/Vol15-issue1/Series-2/J1501026369.pdf (accessed May 5, 2022).
[17]. Kachave, R. N.; Mundhe, A. G. Development and validation of bioanalytical method for determination of nebivolol and valsartan in human plasma by using RP-HPLC. Eur. Pharm. J. 2021, 68, 49-58.
https://doi.org/10.2478/afpuc-2021-0019
[18]. Selvan, P. S.; Gowda, K. V.; Mandal, U.; Solomon, W. D. S.; Pal, T. K. Simultaneous determination of fixed dose combination of nebivolol and valsartan in human plasma by liquid chromatographic-tandem mass spectrometry and its application to pharmacokinetic study. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007, 858, 143-150.
https://doi.org/10.1016/j.jchromb.2007.08.016
[19]. Kamal, A. H.; Marie, A. A.; Hammad, S. F. Validated spectrophotometric methods for simultaneous determination of nebivolol hydrochloride and valsartan in their tablet. Microchem. J. 2020, 155, 104741.
https://doi.org/10.1016/j.microc.2020.104741
[20]. Meselhy, E. M.; Aboul Kheir, A. A.; El Henawee, M. M.; Elmasry, M. S. Simultaneous determination of Nebivolol hydrochloride and Valsartan in their binary mixture using different validated spectro photometric methods. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2020, 230, 118083.
https://doi.org/10.1016/j.saa.2020.118083
[21]. Kokil, S. U.; Bhatia, M. S. Simultaneous estimation of nebivolol hydrochloride and valsartan using RP HPLC. Indian J. Pharm. Sci. 2009, 71, 111-114.
https://doi.org/10.4103/0250-474X.54270
[22]. Samir, A.; Lotfy, H. M.; Salem, H.; Abdelkawy, M. Development and validation of simultaneous spectrophotometric and TLC-spectro densitometric methods for determination of beclomethasone dipropionate and salbutamol in combined dosage form. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2014, 128, 127-136.
https://doi.org/10.1016/j.saa.2014.02.044
[23]. Lotfy, H. M.; Fayez, Y. M.; Tawakkol, S. M.; Fahmy, N. M.; Shehata, M. A. E.-A. Evaluation of graphical and statistical representation of analytical signals of spectrophotometric methods. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2017, 184, 61-70.
https://doi.org/10.1016/j.saa.2017.04.058
[24]. Lotfy, H. M.; Fayez, Y. M.; Tawakkol, S. M.; Fahmy, N. M.; Shehata, M. A. E.-A. Spectrophotometric resolution of the severely overlapped spectra of clotrimazole with dexamethasone in cream dosage form by mathematical manipulation steps. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2018, 202, 115-122.
https://doi.org/10.1016/j.saa.2018.04.072
[25]. Fayez, Y. M.; Tawakkol, S. M.; Fahmy, N. M.; Lotfy, H. M.; Shehata, M. A.-A. Comparative study of the efficiency of computed univariate and multivariate methods for the estimation of the binary mixture of clotrimazole and dexamethasone using two different spectral regions. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2018, 194, 126-135.
https://doi.org/10.1016/j.saa.2018.01.009
[26]. Magdy, R.; Hemdan, A.; Fares, N. V.; Farouk, M. Univariate calibrations with or without chemometric assistance for determination of drugs lacking peak maxima in their zero-order profiles. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2019, 206, 207-215.
https://doi.org/10.1016/j.saa.2018.08.007
[27]. Magdy, R.; Hemdan, A.; Fares, N. V.; Farouk, M. Determination of amlodipine and atorvastatin mixture by different spectrophotometric methods with or without regression equations. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2019, 210, 203-211.
https://doi.org/10.1016/j.saa.2018.11.014
[28]. International conference on harmonization (ICH), Q2B: validation of analytical procedures: methodology US FDA federal register, 1997.
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.13.3.267-272.2272

















European Journal of Chemistry 2022, 13(3), 267-272 | doi: https://doi.org/10.5155/eurjchem.13.3.267-272.2272 | Get rights and content
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Authors

This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at http://www.eurjchem.com/index.php/eurjchem/pages/view/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (http://www.eurjchem.com/index.php/eurjchem/pages/view/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).
© Copyright 2010 - 2023 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2023 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.