European Journal of Chemistry 2023, 14(1), 65-71 | doi: | Get rights and content

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Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy

Aws Maseer Nejres (1,*) orcid , Moath Abdallah Najem (2) orcid

(1) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Mosul, Mosul, 41001, Iraq
(2) Department of Pharmaceutical Chemistry, Faculty of Agriculture and Forestry, University of Mosul, 41001, Iraq
(*) Corresponding Author

Received: 27 Nov 2022 | Revised: 24 Dec 2022 | Accepted: 12 Jan 2023 | Published: 31 Mar 2023 | Issue Date: March 2023


A new fast and simple selective method for the simultaneous determination of lisinopril dihydrate and amlodipine in combined drugs was developed using the fourth derivative spectrum method, based on the zero-crossing-point technique for the determination of compounds in drugs. The wavelength values for lisinopril dihydrate and amlodipine in solvent medium were found to be (203, 207, and 231 nm) and (215, 254, and 277 nm), respectively, with the average obeying Beer’s law in the range of lisinopril dihydrate 2.0 to 45.0 µg/mL and amlodipine 2.0 to 35.0 µg/mL. Lisinopril dihydrate has molar absorptivity regions (9227.76-11700.28 L/, 203 nm), (15320.74-20795.59 L/, 207 nm), and (2207.60-3311.40 L/, 231 nm), while amlodipine (5886.72-10914.96 L/, 215 nm), (5518.8-6418.16 L/, 254 nm) and (1676.08-1921.36 L/, 277 nm). The recovery rate of lisinopril dihydrate in the pharmaceutical dosage forms range was 95.13 to 102.60% and amlodipine 95.14 to 102.80%. The results of the relative error showed that the interferences did not affect the method of estimating these compounds. The proposed method has been successfully applied to estimate pharmaceutical dosage forms.


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European Journal of Chemistry


Amlodipine; Zero-crossing point; Lisinopril dihydrate; Simultaneous determination; Zero-crossing point technique; Fourth derivative spectrum method

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DOI: 10.5155/eurjchem.14.1.65-71.2367

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University of Mosul, Mosul, 41001, Iraq.


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How to cite

Nejres, A.; Najem, M. Eur. J. Chem. 2023, 14(1), 65-71. doi:10.5155/eurjchem.14.1.65-71.2367
Nejres, A.; Najem, M. Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy. Eur. J. Chem. 2023, 14(1), 65-71. doi:10.5155/eurjchem.14.1.65-71.2367
Nejres, A., & Najem, M. (2023). Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy. European Journal of Chemistry, 14(1), 65-71. doi:10.5155/eurjchem.14.1.65-71.2367
Nejres, Aws, & Moath Abdallah Najem. "Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy." European Journal of Chemistry [Online], 14.1 (2023): 65-71. Web. 30 Sep. 2023
Nejres, Aws, AND Najem, Moath. "Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy" European Journal of Chemistry [Online], Volume 14 Number 1 (31 March 2023)

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