European Journal of Chemistry

Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy


Main Article Content

Aws Maseer Nejres
Moath Abdallah Najem


A new fast and simple selective method for the simultaneous determination of lisinopril dihydrate and amlodipine in combined drugs was developed using the fourth derivative spectrum method, based on the zero-crossing-point technique for the determination of compounds in drugs. The wavelength values for lisinopril dihydrate and amlodipine in solvent medium were found to be (203, 207, and 231 nm) and (215, 254, and 277 nm), respectively, with the average obeying Beer’s law in the range of lisinopril dihydrate 2.0 to 45.0 µg/mL and amlodipine 2.0 to 35.0 µg/mL. Lisinopril dihydrate has molar absorptivity regions (9227.76-11700.28 L/, 203 nm), (15320.74-20795.59 L/, 207 nm), and (2207.60-3311.40 L/, 231 nm), while amlodipine (5886.72-10914.96 L/, 215 nm), (5518.8-6418.16 L/, 254 nm) and (1676.08-1921.36 L/, 277 nm). The recovery rate of lisinopril dihydrate in the pharmaceutical dosage forms range was 95.13 to 102.60% and amlodipine 95.14 to 102.80%. The results of the relative error showed that the interferences did not affect the method of estimating these compounds. The proposed method has been successfully applied to estimate pharmaceutical dosage forms.

icon graph This Abstract was viewed 481 times | icon graph Article PDF downloaded 161 times

How to Cite
Nejres, A. M.; Najem, M. A. Simultaneous Determination of Amlodipine and Lisinopril Dihydrate Using Fourth Derivative Spectroscopy. Eur. J. Chem. 2023, 14, 65-71.

Article Details

Crossref - Scopus - Google - European PMC

[1]. Zounr, Z. A. Determination of Lisinopril in pure and tablet form by using 2-hydroxynaphthaldehyde as derivatizing reagent. Pak. J. Anal. Environ. Chem. 2021, 22, 115-126.

[2]. Shulyak, N.; Budzivula, K.; Kucher, T.; Kryskiw, L.; Poliak, O.; Logoyda, L. Spectrophotometric methods for the determination of lisinopril in medicines. Farmatsiia (Sofia) 2021, 68, 811-818.

[3]. Khan, S.; Khan, F. N.; Sadeque, M.; Zainuddin, R.; Zaheer, Z. Development of analytical method and validation for determination of Lisinopril dihydrate in bulk drug and dosage form using HPTLC method. J. Invent. Biomed. Pharm. Sci. 2018, 3, 1-6.

[4]. Yaqoob, S.; Rahim, S.; Bhayo, A. M.; Shah, M. R.; Hameed, A.; Malik, M. I. A novel and efficient colorimetric assay for quantitative determination of amlodipine in environmental, biological and pharmaceutical samples. ChemistrySelect 2019, 4, 10046-10053.

[5]. Courlet, P.; Spaggiari, D.; Desfontaine, V.; Cavassini, M.; Alves Saldanha, S.; Buclin, T.; Marzolini, C.; Csajka, C.; Decosterd, L.-A. UHPLC-MS/MS assay for simultaneous determination of amlodipine, metoprolol, pravastatin, rosuvastatin, atorvastatin with its active metabolites in human plasma, for population-scale drug-drug interactions studies in people living with HIV. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2019, 1125, 121733.

[6]. Darbandi, A.; Sohrabi, M. R.; Bahmaei, M. Development of a chemometric-assisted spectrophotometric method for quantitative simultaneous determination of Amlodipine and Valsartan in commercial tablet. Optik (Stuttg.) 2020, 218, 165110.

[7]. Madhuri, C.; Manohara Reddy, Y. V.; Prabhakar Vattikuti, S. V.; Švorc, Ľ.; Shim, J.; Madhavi, G. Trace-level determination of amlodipine besylate by immobilization of palladium-silver bi-metallic nanoparticles on reduced graphene oxide as an electrochemical sensor. J. Electroanal. Chem. (Lausanne Switz) 2019, 847, 113259.

[8]. Wankhede, S. B.; Khobragade, D. S.; Lote, S. B.; Patil, S. Stability indicating HPTLC method for simultaneous determination of amlodipine besylate and lisinopril in combined dose tablet formulation. Res. J. Pharm. Technol. 2021, 6250-6256.

[9]. Pandya, J. J.; Sanyal, M.; Shrivastav, P. S. Simultaneous densitometric analysis of amlodipine, hydrochlorothiazide, lisinopril, and valsartan by HPTLC in pharmaceutical formulations and human plasma. J. Liq. Chromatogr. Relat. Technol. 2017, 40, 467-478.

[10]. Nejres, A. M.; Ali, H. K.; Behnam, S. P.; Mustafa, Y. F. Potential Effect of Ammonium Chloride on the Optical Physical Properties of Polyvinyl Alcohol. Systematic Reviews in Pharmacy 2020, 11, 726-732.

[11]. Salve, P.; Gharge, D.; Kirtawade, R.; Dhabale, P. N.; Burade, K. B. Simultaneous UV spectrophotometric method for estimation of Amlodipine Besylate and Lisinopril in tablet dosage form. Asian J. Res. Chem. 2010, 3, 201-204.

[12]. Bankar, R. R.; Modha, N. A validated stability indicating RP-HPLC method for estimation of amlodipine besylate and lisinopril in pharmaceutical dosage forms. Research J. Pharm. Tech. 2013, 6, 784-789.

[13]. Prasad, C. V. N.; Saha, R. N.; Parimoo, P. Simultaneous Determination of Amlodipine-Enalapril Maleate and Amlodipine-Lisinopril in Combined Tablet Preparations by Derivative Spectrophotometry. Pharm. Pharmacol. Commun. 1999, 5, 383-388.

[14]. Yadav, N.; Goyal, A. Simultaneous estimation of aliskiren and amlodipine in combined tablet formulation by simultaneous equation and first derivative spectroscopic methods. Org. Med. Chem. Int. J. 2018, 6, 555676.

[15]. Redasani, V. K.; Patel, P. R.; Marathe, D. Y.; Chaudhari, S. R.; Shirkhedkar, A. A.; Surana, S. J. A review on derivative UV-spectrophotometry analysis of drugs in pharmaceutical formulations and biological samples review. J. Chil. Chem. Soc. 2018, 63, 4126-4134.

[16]. Mohammad, M. Y.; Abdullah, M. S.; Sabir, S. S. Simultaneous determination of atenolol and amlodipine using second derivative spectroscopy. Polytech. J. 2019, 9, 25-29.

[17]. Nejres, A. M.; Najem, M. A. A novel Yttrium(III) complex for estimating dopamine in pure and pharmaceutical dosage forms. Biomed. Chem. Sci. 2023, 2, 23-30.

[18]. Rizk, M.; Attia, A. K.; Mohamed, H. Y.; Elshahed, M. Stability indicating HPLC-Fluorescence detection method for the simultaneous determination of linagliptin and empagliflozin in their combined pharmaceutical preparation. Eur. J. Chem. 2021, 12, 168-178.

[19]. Abdelhamid, N. S.; El Aleem, E. A. A. E. A.; Khorshed, A. M.; Amin, M. M. Determination of antihypertensive drugs by using ratio difference spectrophotometric method. Eur. J. Chem. 2019, 10, 12-18.

[20]. Nejres, A.; Najem, M. Use of Mesalazine for the determination of dopamine and its pharmaceutical preparations by spectrophotometric method. Israa University Journal for Applied Science 2022, 6, 228-246.

[21]. Ermer, J. Method validation in pharmaceutical analysis: A guide to best practice; Ermer, J.; McB Miller, J. H., Eds.; Wiley-VCH Verlag: Weinheim, Germany, 2005.

[22]. Christian, G. D.; Dasgupta, P. K.; Schug, K. A. Analytical Chemistry; 7th ed.; John Wiley & Sons: Chichester, England, 2013.

Supporting Agencies

University of Mosul, Mosul, 41001, Iraq.
Most read articles by the same author(s)

Dimensions - Altmetric - scite_ - PlumX

Downloads and views


Download data is not yet available.


Metrics Loading ...
License Terms

License Terms


Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License ( By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License ( are administered by Atlanta Publishing House LLC (European Journal of Chemistry).