European Journal of Chemistry 2011, 2(2), 189-192 | doi: | Get rights and content

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In vitro antimicrobial activity of triterpenoid saponin from Tephrosia purpurea seeds extract

Noor Afshan Khan (1,*)

(1) Department of Postgraduate Studies and Research in Chemistry, Rani Durgawati University, Jabalpur-482001, India
(*) Corresponding Author

Received: 07 Aug 2010 | Revised: 28 Nov 2010 | Accepted: 26 Jan 2011 | Published: 30 Jun 2011 | Issue Date: June 2011


A novel oleanen type triterpenoid glycoside has been isolated from the butanolic extract of the seeds of Tephrosia purpurea. Its structure was elucidated as 3-O-{β-D-glucopyranosyl-(1→6)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-β-D-xylopyranosyl}-2,16-dihydroxy-23,29-dihydroxymethylolean-11,13(18)-diene-28-oic acid on the basis of spectral evidences, i.e. FT-IR, 1H NMR, 13C NMR and FAB-MS data. The isolated saponin was tested for its antimicrobial activity. Significant results were obtained by evaluating the antibacterial activity by “Disc diffusion method” and antifungal activity by “Spore dilution method”. Maximum inhibition was recorded in gram positive bacterium- Streptococcus pneumoniae, while complete inhibition on the growth of fungus-Alternaria alternata was observed at a concentration of 200 μg/mL. The potency of the extract was quantitatively assessed by determining the minimum inhibitory concentration values against selected bacteria. The minimum inhibitory concentration values were in agreement with antibacterial results where minimum value was recorded to be 23 μg/mL for Streptococcus pneumoniae.2_2_189_192_800


Tephrosia purpurea; Spectroscopic analysis; Triterpenoid saponin; Antibacterial activity; Antifungal activity; Minimum Inhibitory Concentration

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DOI: 10.5155/eurjchem.2.2.189-192.239

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How to cite

Khan, N. Eur. J. Chem. 2011, 2(2), 189-192. doi:10.5155/eurjchem.2.2.189-192.239
Khan, N. In vitro antimicrobial activity of triterpenoid saponin from Tephrosia purpurea seeds extract. Eur. J. Chem. 2011, 2(2), 189-192. doi:10.5155/eurjchem.2.2.189-192.239
Khan, N. (2011). In vitro antimicrobial activity of triterpenoid saponin from Tephrosia purpurea seeds extract. European Journal of Chemistry, 2(2), 189-192. doi:10.5155/eurjchem.2.2.189-192.239
Khan, Noor. "In vitro antimicrobial activity of triterpenoid saponin from Tephrosia purpurea seeds extract." European Journal of Chemistry [Online], 2.2 (2011): 189-192. Web. 18 Jan. 2022
Khan, Noor. "In vitro antimicrobial activity of triterpenoid saponin from Tephrosia purpurea seeds extract" European Journal of Chemistry [Online], Volume 2 Number 2 (30 June 2011)

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