European Journal of Chemistry

Statistical analysis in enrichment of total whey protein by continuous foam fractionation method



Main Article Content

Goutam Mukhopadhyay
Jasmina Khanam
Arunabha Nanda
Nripendra Nath Bala
Won Chun Oh

Abstract

The objective of the present study was to optimize the operating conditions in the separation of the total whey proteins from whey by continuous foam fractionation method using response surface methodology (RSM). The effects of the different process variables such as pH (X1) of proteins in feed, gas flow rate, (X2) of initial feed solution, protein: surfactant ratio (X3) and volumetric flow rate (X4) where investigated on the performance criteria of fractionation of raw processed whey. Four factors, three levels Box-Behnken design was used for the optimization procedure. Quadratic model regression equations and response surface plots correlate independent variables (X1, X2, X3 and X4) and dependent variables (response) such as concentration of Foamate (Cf), Enrichment ratio (Er), and percentage Recovery (% Rp) of total whey proteins. All the four factors had significant effects on the response variables. The model predicted that the optimized values of the factors (X1, X2, X3 and X4) such as 5, 290, 1.5 and 14, respectively. The predicted responses were (concentration of foamate, enrichment ratio, and percentage recovery) such as 6647.32, 13.27, and 78.02, respectively. Experiments were performed with the predicted values of factors.

2_4_519_523_800


icon graph This Abstract was viewed 1133 times | icon graph Article PDF downloaded 727 times

How to Cite
(1)
Mukhopadhyay, G.; Khanam, J.; Nanda, A.; Bala, N. N.; Oh, W. C. Statistical Analysis in Enrichment of Total Whey Protein by Continuous Foam Fractionation Method. Eur. J. Chem. 2011, 2, 519-523.

Article Details

Share
Crossref - Scopus - Google - European PMC
References

[1]. Hall, W. L.; Millward, D. J.; Long, S. J.; Morgan, L. M. Brit. J. Nutr. 2003, 89, 239-248.
http://dx.doi.org/10.1079/BJN2002760
PMid:12575908

[2]. Pearce, R. J. Food. Ress. Quartly. 1991, 51, 74-85.

[3]. De Wit, J. N. J. Dairy. Sci. 1998, 81, 597-608.
http://dx.doi.org/10.3168/jds.S0022-0302(98)75613-9

[4]. Pearce, R. J. U. S. Patent. 1995, 5, 331-340.

[5]. Fuda, E.; Bhatia, D.; Pyle, D. L.; Jauregi. P. Biotech. Bioeng. 2005, 90, 531-542.

[6]. Hossain, Md. M.; Saleh, Z. Aust. Biotech. 2001, 11, 29-31.

[7]. Brown, A. K.; Kaul, A.; Varley, J. Biotech Bioeng., 1999, 62, 291-299.
http://dx.doi.org/10.1002/(SICI)1097-0290(19990205)62:3<291::AID-BIT5>3.0.CO;2-T

[8]. Mass, K. Adsorptive Bubble Separation Technique, Academic Press, New York, 1974, 165-167.

[9]. Uraizee, F.; Nrsimhan, G. Engyme. Microb. Technol. 1990, 12, 315-316.
http://dx.doi.org/10.1016/0141-0229(90)90105-Y

[10]. Lemlich, R. Adsorptive Bubble Separation Technique, Academic Press, New York, 1972, 133-143.

[11]. Maruyama, H.; Suzuki, A.; Inoue, N. Biochem. Engin. 2006, 30, 253-259.
http://dx.doi.org/10.1016/j.bej.2006.05.004

[12]. Suzuki, A.; Yasuhara, K.; Seki, H.; Maruyama, H. J. Colloid and Interface Sci. 2002, 253, 402-408.
http://dx.doi.org/10.1006/jcis.2002.8526
PMid:16290871

[13]. Guerrero, S.; Alzamora, S. M.; Gerschenson, L. N. J. Food. Eng. 1996, 28, 307-322.
http://dx.doi.org/10.1016/0260-8774(95)00036-4

[14]. Collar, C.; Martinez, C. S. J. Food. Sci. 1993, 58, 1324-1328.
http://dx.doi.org/10.1111/j.1365-2621.1993.tb06175.x

[15]. De la Maza, A.; Marich, A. M.; Coderch, L.; Baucells, J.; Parra, J. L. Colloids Surfaces A 1996, 113, 259-267.
http://dx.doi.org/10.1016/0927-7757(96)03619-9

[16]. Shaoo, B. K.; Gowda, V.; Ghosh. A.; Chatterjee, B.; Bose, A.; Chakraborty, U.; Das, A.; Pal, T. K. Pharm. Ind. 2009, 71, 1423-1428.

[17]. Boea, A.; Leueuta, S. E. Drug Dev. Ind. Pharm. 1998, 24, 145-155.
http://dx.doi.org/10.3109/03639049809085599
PMid:15605444

[18]. Fuda, E.; Jauregi, P.; Pyle, D. L. J. Collod. Int. Sci. 2004, 20, 514-525.

[19]. Brown, A. K.; Kaul, A.; Varley, J. Biotech Bioeng. 1999, 62, 278-290.
http://dx.doi.org/10.1002/(SICI)1097-0290(19990205)62:3<278::AID-BIT4>3.0.CO;2-D

[20]. Saleh, Z. S.; Hossain, Md. M. Chem. Eng. Process. 2001, 40, 371-378.
http://dx.doi.org/10.1016/S0255-2701(01)00115-5

[21]. Du, L.; Prokop, A.; Tanner, R. D. Appl. Biochem. Biotech. 2002, 98-100, 1075-1091.
http://dx.doi.org/10.1385/ABAB:98-100:1-9:1075

[22]. Ekici, P.; Backleh-Sohrt, M.; Parlar, H. J. Food. Sci. Nutria. 2005, 56, 223-229.

[23]. Ranghavan, C. V.; Babu, R. S.; Chand, N.; Rao, P. N. S. J. Food. Sci. Tech. 1996, 33, 313-321.

Most read articles by the same author(s)
TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).