OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity
Nehal Fayek Farid (1) , Ibrahim Ahmed Naguib (2) , Radwa Saeed Moatamed (3) , Mohamed Refaat El-Ghobashy (4,*)
(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(3) National Organization for Drug Control and Research (NODCAR), Giza, 11843, Egypt
(4) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt
(*) Corresponding Author
Received: 03 Mar 2016 | Accepted: 02 Apr 2016 | Published: 30 Jun 2016 | Issue Date: June 2016
A new sensitive, simple, rapid, accurate and precise spectrofluorimetric method for determination of diflunisal and its impurity is developed. Determination of diflunisal is based on first derivative spectrofluorimetric method, while its impurity can be determined by zero order spectrofluorimetric method. Diflunisal was measured at zero-crossing wavelength 394nm (zero crossing point with its impurity) which was selected for quantification of diflunisal. The impurity was measured directly at 334 nm, using 0.05 M phosphate buffer (pH = 9) as solvent. The analytical signal resulting from first derivative and zero order spectra were measured for diflunisal and its impurity, respectively. Linearity was over the range of 0.1-0.9 μg/mL for both with detection limit of 0.02 and 0.03 μg/mL and quantitation limit of 0.07 and 0.09 μg/mL for diflunisal and its impurity, respectively. The proposed method was validated as per ICH guidelines.The accuracy was checked by applying the proposed method for the determination of the drug and its impurity, the mean percentage recoveries were found to be 99.61±0.911 and 100.41±1.373 for diflunisal and its impurity, respectively. RSD values for repeatability testing were 0.268 and 0.569 and for intermediate precision were 0.224 and 0.259 for diflunisal and its impurity, respectively. The proposed method was effectively applied to analysis of studied drug in its tablet formulation. The results obtained by it were statistically compared with the reported method revealing high accuracy and good precision.
Links for Article
| | | | | | |
| | | | | | |
Article MetricsThis Abstract was viewed 691 times | PDF Article downloaded 251 times
Rameda Pharmaceutical Company, Egypt
. Nehal Fayek Farid, Nada Sayed Abdelwahab
Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance
European Journal of Chemistry 7(3), 329, 2016
. The British Pharmacopoeia. Her Majesty's Stationery Office, London, 2010, pp. 2606-2610, 2615.
. Maher, H. M. J. Liq. Chrom. Tech. 2014, 37, 634-652.
. Shaalan, R. A.; Belal, T. S. Sci. Pharm. 2013, 81, 713-731.
. Wahbi, A.; Mabrouk, M. M.; Moneeb, M. S. Pak. J. Pharm. Sci. 2009, 22, 8-17.
. El-Saharty, Y.; Refaat, M.; El-Khateeb, S. Bull. Fac. Pharm. Cairo Univ. 2002, 40, 47-64.
. Streete, P. Biomed. Sci. Appl. 1989, 495, 179-193.
. Bebawy, L; Elkousy, N. Pharm. Biomed. Anal. 1999, 20, 663-670.
. Haj, B. M; El-Arini, A. M.; Hassan, M. H.; Khadem, R. K.; Marzouq, M. S. Foren. Sci. Inter. 1999, 105, 141-53.
. Srinivas, B; Yadagiriswamy, P.; Venkateswarlu, G. Inter. J. Pharm. Sci. Res. 2015, 6, 1002-1010.
. Sayin, F.; Kir, S. FABAD J. Pharm. Sci. 2004, 29, 121-126.
. Murillo-Pulgarin, J. A; Alanon-Molina, A. Appl. Spectrosc. 2010, 64, 949-55.
. Ioannou, P. C.; Lianidou, E. S.; Konstantianos, D. G. Anal. Chim. Acta 1995, 300, 237-241.
. Pulgarin, M.; Molina, A.; Lopez, F. P. Anal. Chim. Acta 2007, 583, 55-62.
. Neeli, S. A.; Anuganti, H.; Sathesh, R.; Subrahmanyam, V. S. Dhaka Univ. J. Pharm. Sci. 2013, 12(1), 35-40.
. Murillo, J.; Garcia, L. Anal. Lett. 1996, 29, 423-438.
. Dress, J. C.; Wu, A. H. B. Analytical Techniques, Wolters Kluwer Vitalstream, 2008, Chapter 5, pp. 140.
. ICH, Q. R., Validation of Analytical Procedures: Text and Methodology, ICH Harmonized Tripartite Guideline, 2005.
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.7.2.201-205.1421
| | | | | | |
| | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2016, 7(2), 201-205 | doi: https://doi.org/10.5155/eurjchem.7.2.201-205.1421 | Get rights and content
- There are currently no refbacks.
© Copyright 2010 - 2020 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2020 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.