European Journal of Chemistry 2019, 10(3), 263-266 | doi: https://doi.org/10.5155/eurjchem.10.3.263-266.1825 | Get rights and content

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Application of multiple reaction monitoring for quantitation of sweeteners in food products


Wafaa Abdou Zaghary (1) orcid , Ahmed Mostafa Abdalla (2,*) orcid , Emily Tawfik Hanna (3) orcid , Nermeen Abdallah Shoukry (4) orcid

(1) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt
(2) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt
(3) Nutrition Biochemistry and Metabolism Department, National Nutrition Institute, Cairo, 11562, Egypt
(4) Nutrition Biochemistry and Metabolism Department, National Nutrition Institute, Cairo, 11562, Egypt
(*) Corresponding Author

Received: 08 Dec 2018 | Revised: 15 May 2019 | Accepted: 17 May 2019 | Published: 30 Sep 2019 | Issue Date: September 2019

Abstract


A coupled UPLC-MS/MS method has been developed and validated for the simultaneous quantitation of food sweeteners stevioside (STV) and aspartame (ASP). Good chromato-graphic separation was achieved on a Hypersil gold 50×2.1 mm (1.9 μm) column, using a gradient flow of 10 mM ammonium acetate, pH = 2.9 adjusted with formic acid, and 10 mM ammonium acetate in acetonitrile:water (95:5, v:v) as the mobile phase. The eluate was introduced to ESI-Mass spectrometer and scanned using multiple reaction monitoring (MRM). The method was robust, reproducible and easy to use and was validated according to ICH guidelines for the accuracy and precision giving acceptable ranges. The utilization of multiple reaction monitoring improved the selectivity of detection. Method was linear in the ranges of 2-250 ng/mL for STV and ASP. Application of this method on laboratory mixtures of the selected sweeteners was successful. The using of mass spectrometry make the method selective and measurement did not affect the presence of impurities, additive and other ingredients of detection due to the simplicity and sensitivity of this method allows the utilization of method in quality control of STV and ASP.


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European Journal of Chemistry

Keywords


UPLC; Aspartam; Stevioside; Sweeteners; Multiple reaction monitoring; Tandem mass spectrometric technique

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DOI: 10.5155/eurjchem.10.3.263-266.1825

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Funding information


The Pharmaceutical Services Centre and LC-MS Unit, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.

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How to cite


Zaghary, W.; Abdalla, A.; Hanna, E.; Shoukry, N. Eur. J. Chem. 2019, 10(3), 263-266. doi:10.5155/eurjchem.10.3.263-266.1825
Zaghary, W.; Abdalla, A.; Hanna, E.; Shoukry, N. Application of multiple reaction monitoring for quantitation of sweeteners in food products. Eur. J. Chem. 2019, 10(3), 263-266. doi:10.5155/eurjchem.10.3.263-266.1825
Zaghary, W., Abdalla, A., Hanna, E., & Shoukry, N. (2019). Application of multiple reaction monitoring for quantitation of sweeteners in food products. European Journal of Chemistry, 10(3), 263-266. doi:10.5155/eurjchem.10.3.263-266.1825
Zaghary, Wafaa, Ahmed Mostafa Abdalla, Emily Tawfik Hanna, & Nermeen Abdallah Shoukry. "Application of multiple reaction monitoring for quantitation of sweeteners in food products." European Journal of Chemistry [Online], 10.3 (2019): 263-266. Web. 23 Sep. 2023
Zaghary, Wafaa, Abdalla, Ahmed, Hanna, Emily, AND Shoukry, Nermeen. "Application of multiple reaction monitoring for quantitation of sweeteners in food products" European Journal of Chemistry [Online], Volume 10 Number 3 (30 September 2019)

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