Synthesis and characterization of 2-formylthymol-based azo-aldehyde dyes: Probing their efficacy as a radical scavenger in antioxidant applications

Kishor Jaysing Girase; Santosh Venkatrao Dandge; Mangal Arun Chaudhari; Ratnamala Subhash Bendre

doi:10.5155/eurjchem.16.3.319-326.2687

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Published: Sep 30, 2025

DOI: https://doi.org/10.5155/eurjchem.16.3.319-326.2687

Keywords:

α-Amylase, Antioxidants, Diazotization, 2-Formylthymol, Azo-aldehyde dye, Reimer-Tiemann reaction

Section

Research Article

Issue

Vol. 16 No. 3 (2025): September 2025

Dates

Received 2025-04-07
Accepted 2025-07-04
Published 2025-09-30

Kishor Jaysing Girase

School of Chemical Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, 425001, India

https://orcid.org/0009-0008-5228-9498

Santosh Venkatrao Dandge

School of Chemical Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, 425001, India

https://orcid.org/0009-0000-2993-127X

Mangal Arun Chaudhari

School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon Maharashtra, 425001, India

https://orcid.org/0009-0006-6447-1938

Ratnamala Subhash Bendre

School of Chemical Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, 425001, India

https://orcid.org/0000-0001-6987-9912

Abstract

A new series of azo-aldehyde dyes has been derived by performing a diazocoupling reaction between 2-isopropyl-5-methyl phenol and diazonium salts obtained by diazotization of differently substituted aromatic amines. The structures of newly synthesized azo-aldehydes were confirmed by modern analytical spectroscopic techniques such as ¹H NMR, ¹³C NMR, FT-IR, and Mass. Thereafter, all synthesized azo-aldehyde dyes were evaluated for their in vitro antibacterial activity against S. aureus, S. typhumurium, E. coli, and P. aeruginosa strains using the plate method. The formed compounds were also evaluated for antioxidant activity. The present results provide new data to support that the thymol-based azo-aldehyde dyes have a potential to explore a variety of applications in the modern field of molecules and materials of high biological relevance.

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Girase, K. J.; Dandge, S. V.; Chaudhari, M. A.; Bendre, R. S. Synthesis and Characterization of 2-Formylthymol-Based Azo-Aldehyde Dyes: Probing Their Efficacy As a Radical Scavenger in Antioxidant Applications. Eur. J. Chem. 2025, 16, 319-326.

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