European Journal of Chemistry

Synthesis and characterization of 2-formylthymol-based azo-aldehyde dyes: Probing their efficacy as a radical scavenger in antioxidant applications

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Published: Sep 30, 2025
DOI: https://doi.org/10.5155/eurjchem.16.3.319-326.2687
Keywords:
α-Amylase, Antioxidants, Diazotization, 2-Formylthymol, Azo-aldehyde dye, Reimer-Tiemann reaction
Section
Research Article
Issue
Vol. 16 No. 3 (2025): September 2025
Dates
Received 2025-04-07
Accepted 2025-07-04
Published 2025-09-30
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Kishor Jaysing Girase
School of Chemical Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, 425001, India
https://orcid.org/0009-0008-5228-9498
Santosh Venkatrao Dandge
School of Chemical Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, 425001, India
https://orcid.org/0009-0000-2993-127X
Mangal Arun Chaudhari
School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon Maharashtra, 425001, India
https://orcid.org/0009-0006-6447-1938
Ratnamala Subhash Bendre
School of Chemical Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, 425001, India
https://orcid.org/0000-0001-6987-9912

Abstract

A new series of azo-aldehyde dyes has been derived by performing a diazocoupling reaction between 2-isopropyl-5-methyl phenol and diazonium salts obtained by diazotization of differently substituted aromatic amines. The structures of newly synthesized azo-aldehydes were confirmed by modern analytical spectroscopic techniques such as 1H NMR, 13C NMR, FT-IR, and Mass. Thereafter, all synthesized azo-aldehyde dyes were evaluated for their in vitro antibacterial activity against S. aureus, S. typhumurium, E. coli, and P. aeruginosa strains using the plate method. The formed compounds were also evaluated for antioxidant activity. The present results provide new data to support that the thymol-based azo-aldehyde dyes have a potential to explore a variety of applications in the modern field of molecules and materials of high biological relevance.


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Girase, K. J.; Dandge, S. V.; Chaudhari, M. A.; Bendre, R. S. Synthesis and Characterization of 2-Formylthymol-Based Azo-Aldehyde Dyes: Probing Their Efficacy As a Radical Scavenger in Antioxidant Applications. Eur. J. Chem. 2025, 16, 319-326.

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