European Journal of Chemistry 2023, 14(1), 114-120 | doi: https://doi.org/10.5155/eurjchem.14.1.114-120.2395 | Get rights and content

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Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand


Ananya Bahuguna (1) orcid , Shiv Kumar Dubey (2,*) orcid , Vaishali Garia (3) orcid , Ravendra Kumar (4) orcid , Om Prakash (5) orcid , Dharmendra Singh Rawat (6) orcid

(1) Department of Biochemistry, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
(2) Department of Biochemistry, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
(3) Department of Biochemistry, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
(4) Department of Chemistry, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
(5) Department of Chemistry, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
(6) Department of Biological Sciences, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
(*) Corresponding Author

Received: 23 Dec 2022 | Revised: 18 Jan 2023 | Accepted: 25 Jan 2023 | Published: 31 Mar 2023 | Issue Date: March 2023

Abstract


The current study describes the analysis of the phytochemical composition and biological activities of various polarity extracts of the Anaphalis busua plant that was collected at an altitude of 1654 m in the Himalayan terrain of Uttarakhand, India. The extracts were prepared by the cold percolation method, which was then subjected to GC-MS for phytochemical analysis. A total of 31 compounds were identified that constituted 94.95% of the total methanolic extract. Mome inositol (31.03%) was identified as the main compound in the methanolic extract. Twenty-two compounds that comprise 68.24% of the total hexane extract were identified. Tetracontane (19.33%) was present in a significant proportion. The methanolic extract demonstrated potent antioxidant activity in terms of DPPH radical scavenging and metal chelating activity that have IC50 values of 81.71±1.334 and 11.26±0.005 µg/mL, respectively, compared to standards ascorbic acid and EDTA that have IC50 values at 12.71±0.02 and 11.36±0.06 µg/mL, respectively. The methanolic extract showed potent anti-inflammatory activity with an IC50 value of 24.10±0.09 µg/mL in comparison to standard diclofenac potassium with an IC50 value of 18.95±0.03 µg/mL. In vitro studies reveal that A. busua has a strong therapeutic potential and, if further explored, may prove to be a powerful antioxidant, anti-inflammatory, and cost-effective agent compared to synthetically derived agents from pharmaceutical industries.


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Editor-in-Chief
European Journal of Chemistry

Keywords


Anaphalis busua; Biological activity; Mass spectrometry; Antioxidant activity; Gas chromatography; Anti-inflammatory activity

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DOI: 10.5155/eurjchem.14.1.114-120.2395

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Funding information


The Advanced Instrumentation Research Facility of Jawaharlal Nehru University (JNU), New Delhi, India and The Department of Biochemistry, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India.

Citations

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[1]. Vaishali Garia, Shiv Kumar Dubey, Ananya Bahuguna, Ravendra Kumar, Om Prakash, Dharmendra Singh Rawat
Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Artemisia nilagirica collected from hilly areas in the Himalayan terrain of Uttarakhand
European Journal of Chemistry  14(3), 323, 2023
DOI: 10.5155/eurjchem.14.3.323-329.2449
/


References


[1]. Hussein, R.; A. El-Anssary, A. Plants secondary metabolites: The key drivers of the pharmacological actions of medicinal plants, https://www.intechopen.com/chapters/61866. In Herbal Medicine; IntechOpen, 2019.
https://doi.org/10.5772/intechopen.76139

[2]. Chemistry of phytopotentials: Health, energy and environmental perspectives; Khemani, L. D.; Srivastava, M. M.; Srivastava, S., Eds.; Springer Berlin Heidelberg: Berlin, Heidelberg, 2012.

[3]. Gairola, K.; Gururani, S.; Kumar, R.; Prakash, O.; Agarwal, S.; Dubey, S. K. Phytochemical composition, antioxidant, and anti-inflammatory activities of essential oil of Acmella uliginosa (Sw.) Cass. grown in North India Terai region of Uttarakhand. Trends Phytochem. Res. 2021, 5, 44-52.
https://doi.org/10.1590/s2175-97902022e20353

[4]. Jamshidi-Kia, F.; Lorigooini, Z.; Amini-Khoei, H. Medicinal plants: Past history and future perspective. J. Herbmed Pharmacol. 2018, 7, 1-7.
https://doi.org/10.15171/jhp.2018.01

[5]. Raghuwanshi, S.; Negi, H.; Aggarwal, T.; Zaidi, M.; Goel, R. Comparative biodegradation studies of cow dung modified epoxy with epoxy using an indigenously developed bacterial consortium. Afr. J. Microbiol. Res. 2015, 9, 1558-1572.
https://doi.org/10.5897/AJMR2015.7462

[6]. Mujtaba, M.; Yilmaz, B. A.; Cansaran-Duman, D.; Akyuz, L.; Yangın, S.; Kaya, M.; Çeter, T.; Khawar, K. M. Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay. Polym. Bull. (Berl.) 2022, 79, 519-540.
https://doi.org/10.1007/s00289-020-03531-7

[7]. Natural product experiments in drug discovery; Arunachalam, K.; Yang, X.; Puthanpura Sasidharan, S., Eds.; Springer US: New York, NY, 2023.

[8]. Gairola, K.; Gururani, S.; Kumar, R.; Prakash, O.; Agrawal, S.; Dubey, S. K. Composition, Antioxidant and Anti-inflammatory activities of Hexane and Methanol extracts of Acmella uliginosa from Terai region of Uttarakhand. Braz. J. Pharm. Sci. 2022, 58.
https://doi.org/10.1590/s2175-97902022e20353

[9]. Prasad, K. Phytochemical analysis and antioxidant activity essential oil of Anaphalis contorta from Uttrakhand Himalayas. J. Anal. Pharm. Res. 2017, 6.
https://doi.org/10.15406/japlr.2017.06.00172

[10]. Rajbhandari, M.; Lindequist, U. Isolation of Flavonoids from Anaphalis busua and their Antibacterial Activity. J. Inst. Sci. Technol. 2020, 25, 1-6.
https://doi.org/10.3126/jist.v25i1.29415

[11]. Kumar, R.; Kumar, R.; Anjum, B.; Prakash, O.; Joshi, A.; Pant, A. K.; Patel, C.; Srivastava, R. M. Phytochemical analysis, in-vitro antioxidant, anti-inflammatory and insect antifeeding activity of methanolic extract of Limnophila indica (L.) Druce. Int. J. Chem. Stud. 2019, 7, 1691-1696.

[12]. Adams, R. P. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry; Allured Publishing Corporation, 2007.

[13]. Huang, H.-C.; Wang, H.-F.; Yih, K.-H.; Chang, L.-Z.; Chang, T.-M. The dual antimelanogenic and antioxidant activities of the essential oil extracted from the leaves of Acorus macrospadiceus (Yamamoto) F. N. Wei et Y. K. Li. Evid. Based. Complement. Alternat. Med. 2012, 2012, 781280.
https://doi.org/10.1155/2012/781280

[14]. Pavithra, K.; Vadivukkarasi, S. Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Sci. Hum. Wellness 2015, 4, 42-46.
https://doi.org/10.1016/j.fshw.2015.02.001

[15]. Yen, G. Antioxidant activity of anthraquinones and anthrone. Food Chem. 2000, 70, 437-441.
https://doi.org/10.1016/S0308-8146(00)00108-4

[16]. Kar, B.; Kumar, R. B. S.; Karmakar, I.; Dola, N.; Bala, A.; Mazumder, U. K.; Hadar, P. K. Antioxidant and in vitro anti-inflammatory activities of Mimusops elengi leaves. Asian Pac. J. Trop. Biomed. 2012, 2, S976-S980.
https://doi.org/10.1016/S2221-1691(12)60346-3

[17]. Amarowicz, R.; Pegg, R. B.; Rahimi-Moghaddam, P.; Barl, B.; Weil, J. A. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 2004, 84, 551-562.
https://doi.org/10.1016/S0308-8146(03)00278-4

[18]. Brand-Williams, W.; Cuvelier, M. E.; Berset, C. Use of a free radical method to evaluate antioxidant activity. Lebenson. Wiss. Technol. 1995, 28, 25-30.
https://doi.org/10.1016/S0023-6438(95)80008-5

[19]. Ebrahimzadeh, M. A.; Pourmorad, F.; Bekhradnia, A. R. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr. J. Biotechnol. 2008, 7, 3188-3192.

[20]. Das, S.; Vasudeva, N.; Sharma, S. Chemical composition of ethanol extract of Macrotyloma uniflorum (Lam.) Verdc. using GC-MS spectroscopy. Org. Med. Chem. Lett. 2014, 4, 13.
https://doi.org/10.1186/s13588-014-0013-y

[21]. Swamy, M. K.; Arumugam, G.; Kaur, R.; Ghasemzadeh, A.; Yusoff, M. M.; Sinniah, U. R. GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves. Evid. Based. Complement. Alternat. Med. 2017, 2017, 1517683.
https://doi.org/10.1155/2017/1517683

[22]. Gülçin, İ. Fe(3+)-Fe(2+) transformation method: an important antioxidant assay. Methods Mol. Biol. 2015, 1208, 233-246.
https://doi.org/10.1007/978-1-4939-1441-8_17

[23]. Kamila, P. K.; Bal, P.; Ray, A.; Kar, S. K.; Panda, P. C. Nutritional value, phytochemical composition and antioxidant potential of the seed flour of Cycas sphaerica, endemic to India. S. Afr. J. Bot. 2022, 150, 965-973.
https://doi.org/10.1016/j.sajb.2022.08.021

[24]. Arora, S.; Meena, S. Pharmagnostic potentialities of Ceropegia bulbosa roxb. Var. Lushii (grah.) Hook.f.: an endangered plant from Thar desert, Rajasthan, India. Int. Res. J. Pharm. 2017, 8, 77-82.
https://doi.org/10.7897/2230-8407.086100

[25]. Gururani, S.; Gairola, K.; Kumar, R.; Prakash, O.; Dubey, S. K. Altitudinal and geographical variations in phytochemical composition and biological activities of Curcuma longa accession from Uttarakhand, the Himalayan region. J. Food Process. Preserv. 2022, 46, e16384.
https://doi.org/10.1111/jfpp.16384

[26]. Tahiri, S.; Fulgence, M. K.; Kouame, D. B. Phytochemical analysis, investigation of antioxidant and anti-inflammatory activities of ethanolic and aqueous extracts of roots of Combretum glutinosum Perr. ex DC from Cote d'Ivoire. Eur. J. Chem. 2022, 13, 478-482.
https://doi.org/10.5155/eurjchem.13.4.478-482.2352

[27]. Swamy, M. K.; Sinniah, U. R. A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.: An aromatic medicinal plant of industrial importance. Molecules 2015, 20, 8521-8547.
https://doi.org/10.3390/molecules20058521


How to cite


Bahuguna, A.; Dubey, S.; Garia, V.; Kumar, R.; Prakash, O.; Rawat, D. Eur. J. Chem. 2023, 14(1), 114-120. doi:10.5155/eurjchem.14.1.114-120.2395
Bahuguna, A.; Dubey, S.; Garia, V.; Kumar, R.; Prakash, O.; Rawat, D. Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand. Eur. J. Chem. 2023, 14(1), 114-120. doi:10.5155/eurjchem.14.1.114-120.2395
Bahuguna, A., Dubey, S., Garia, V., Kumar, R., Prakash, O., & Rawat, D. (2023). Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand. European Journal of Chemistry, 14(1), 114-120. doi:10.5155/eurjchem.14.1.114-120.2395
Bahuguna, Ananya, Shiv Kumar Dubey, Vaishali Garia, Ravendra Kumar, Om Prakash, & Dharmendra Singh Rawat. "Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand." European Journal of Chemistry [Online], 14.1 (2023): 114-120. Web. 8 Dec. 2023
Bahuguna, Ananya, Dubey, Shiv, Garia, Vaishali, Kumar, Ravendra, Prakash, Om, AND Rawat, Dharmendra. "Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand" European Journal of Chemistry [Online], Volume 14 Number 1 (31 March 2023)

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